![Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - Analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-1F1-Western-Blot-H00001415-M02-img0005.jpg "Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - Analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | CRYBB2 (NP_000487.1, 1 a.a. ~ 205 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN |
| Specificity | This product is specific for Human CRYBB2 monoclonal antibody (M02), clone 1F1 [Gene ID: 1415]. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CRYBB2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF was reported in scientific literature (PMID: 31511085). |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00001415-M02 | Applications | Species |
|---|---|---|
| Barrow MA, Martin ME, Coffey A et al. A functional role for the cancer disparity-linked genes, CRY beta B2 and CRY beta B2P1, in the promotion of breast cancer Breast Cancer Res. 2019-09-11 [PMID: 31511085] (ICC/IF, Human) | ICC/IF | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 11/25/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CRYBB2 |
| Uniprot |
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![Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - analysis of CRYBB2 in stably transfected HEK293T cell lysate. Note: membrane was blocked overnight in 3% BSA in TBST at 4C to reduce the background. Image from verified customer review.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-1F1-Western-Blot-H00001415-M02-img0003.jpg "Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - analysis of CRYBB2 in stably transfected HEK293T cell lysate. Note: membrane was blocked overnight in 3% BSA in TBST at 4C to reduce the background. Image from verified customer review.")
![ELISA: CRYBB2 Antibody (1F1) [H00001415-M02] - Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-1F1-ELISA-H00001415-M02-img0006.jpg "ELISA: CRYBB2 Antibody (1F1) [H00001415-M02] - Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.")
