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Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free

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Western Blot: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha-parvin.
Immunocytochemistry/ Immunofluorescence: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Alpha-parvin/Actopaxin.
Immunohistochemistry: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of extracts (35ug) from 9 different cell lines by using PARVA monoclonal antibody (HepG2: human; HeLa: human; SVT2: ...read more
Immunocytochemistry/ Immunofluorescence: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of HeLa cells.
Immunohistochemistry: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free [NBP2-72018] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PARVA(NP_060692) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PARVA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:4000
Theoretical MW
42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Alpha-parvin/Actopaxin Antibody (OTI1C10) - Azide and BSA Free

  • Actopaxin
  • alphaParvin
  • alpha-Parvin
  • Calponin-like integrin-linked kinase-binding protein
  • CH-ILKBP
  • FLJ10793
  • FLJ12254
  • FLJ25106
  • FLJ46049
  • Matrix-remodeling-associated protein 2
  • matrix-remodelling associated 2
  • MXRA2
  • PARVA
  • parvin, alpha

Background

Members of the parvin family, including Actopaxin, are actin-binding proteins associated with focal contacts.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PARVA