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Mouse alpha 1-Microglobulin ELISA Kit (Colorimetric)

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ELISA: Mouse alpha 1-Microglobulin ELISA Kit (Colorimetric) [NBP3-00457] - Samples were spiked with high concentrations of Mouse alpha 1-Microglobulin and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Mouse alpha 1-Microglobulin ELISA Kit (Colorimetric) [NBP3-00457] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

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Mouse alpha 1-Microglobulin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse alpha 1-Microglobulin in samples. No significant cross-reactivity or interference between Mouse alpha 1-Microglobulin and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.19%
Intra-Assay
5.01%
Spike Recovery
87 - 108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
AMBP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse alpha 1-Microglobulin ELISA Kit (Colorimetric)

  • A1M
  • alpha 1Microglobulin
  • alpha 1-Microglobulin
  • alpha-1-microglobulin/bikunin precursor
  • AMBP
  • bikunin
  • EDC1
  • HCP
  • HCPcomplex-forming glycoprotein heterogeneous in charge
  • HI30
  • IATIL
  • inter-alpha-trypsin inhibitor light chain
  • ITI
  • ITIL
  • ITILC
  • ITILgrowth-inhibiting protein 19
  • protein AMBP
  • protein HC
  • trypstatin
  • uristatin
  • uronic-acid-rich protein
  • UTI

Background

Alpha-1-Microglobulin encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AMBP