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ALG12 Recombinant Protein Antigen

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Summary
Reactivity HuSpecies Glossary
Applications AC

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ALG12 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ALG12.

Source: E. coli

Amino Acid Sequence: KVEESFNLQATHDLLYHWQDLEQYDHLEF

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
ALG12
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-58353.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for ALG12 Recombinant Protein Antigen

  • alpha-1,6-mannosyltransferase)
  • asparagine-linked glycosylation 12 homolog (S. cerevisiae
  • asparagine-linked glycosylation 12 homolog (yeast
  • asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S.cerevisiae)
  • Asparagine-linked glycosylation protein 12 homolog
  • CDG1G
  • dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase
  • dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase
  • EC 2.4.1.-
  • ECM39
  • hALG12
  • Mannosyltransferase ALG12 homolog
  • Membrane protein SB87
  • MGC111358
  • MGC3136
  • PP14673

Background

ALG12 encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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ALG12 Recombinant
Protein Antigen
NBP2-58353PEP
ALG12 Recombinant Protein Antigen
Species: Hu
Applications: AC

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Bioinformatics

Gene Symbol ALG12