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PIGB Antibody

Images

 
Western Blot: PIGB Antibody [NBP1-85856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PIGB Antibody [NBP1-85856] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Western Blot: PIGB Antibody [NBP1-85856] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PIGB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QRGTLDVMSHIQKVCYNNPNKSSASIFIMMPCHSTPYYSHVHCPLPMRFLQCPPDLTGKSHYLDEAD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIGB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PIGB Protein (NBP1-85856PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIGB Antibody

  • EC 2.4.1
  • EC 2.4.1.-
  • GPI mannosyltransferase 3
  • GPI mannosyltransferase III
  • GPI-MT-III
  • MGC21236
  • phosphatidylinositol glycan anchor biosynthesis, class B
  • phosphatidylinositol glycan, class B
  • Phosphatidylinositol-glycan biosynthesis class B protein
  • PIG-B

Background

PIGB, also referred to as GPI mannosyltransferase 3, is 554 amino acids long and is approximately 65 kDa. PIGB is a mannosyltransferase that is implicated in the biosynthesis of GPI anchors. Current research on PIGB is being performed relating to several diseases and disorders including malaria, sleeping sickness, Griscelli syndrome and encephalomalacia. PIGB has also been shown to have interactions with TMED10, CDC40, DNAJA1, FNBP1L and EEF1A1 in pathways such as GPI anchor biosynthesis and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PIGB