Novus Biologicals products are now on bio-techne.com

Recombinant Human PIGZ GST (N-Term) Protein

Images

 
12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

Recombinant Human PIGZ GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-579 of Human PIGZ full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLLRVLWCLLPQTGYVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGSTFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALALLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGGIVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATDSWYFSSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQALQAAWQQLQVGLQASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFLIPLLVPLVLLCSPQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPVLPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGTEDWALCQTLKSFTRQPACQVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLLSGAWRDHLSLHIVELGEET

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Full Length Recombinant Protein
Gene
PIGZ
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
89.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human PIGZ GST (N-Term) Protein

  • class Z
  • EC 2.4.1
  • EC 2.4.1.-
  • FLJ12768
  • GPI mannosyltransferase 4
  • GPI mannosyltransferase IV
  • GPI-MT-IV
  • hSMP3
  • phosphatidylinositol glycan anchor biosynthesis, class Z
  • Phosphatidylinositol-glycan biosynthesis class Z protein
  • PIG-Z
  • SMP3 mannosyltransferase
  • SMP3SMP3 homolog

Background

The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85856
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-02541
Species: Hu, Pm, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24653
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-62449
Species: Hu
Applications: IHC, IHC-P, WB
AF4064
Species: Mu
Applications: ICC, WB
NBP2-94070
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00114569-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-90191
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-02860
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-56262
Species: Hu
Applications: ICC/IF
NBP2-39092
Species: Hu
Applications: IHC, IHC-P
NBP2-13760
Species: Hu
Applications: IHC, IHC-P
NBP1-83810
Species: Hu
Applications: IHC, IHC-P
NBP1-53181
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-81800
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-90235
Species: ChHa, Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB

Publications for PIGZ Recombinant Protein (H00080235-P01) (0)

There are no publications for PIGZ Recombinant Protein (H00080235-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PIGZ Recombinant Protein (H00080235-P01) (0)

There are no reviews for PIGZ Recombinant Protein (H00080235-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PIGZ Recombinant Protein (H00080235-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PIGZ Products

Research Areas for PIGZ Recombinant Protein (H00080235-P01)

Find related products by research area.

Blogs on PIGZ

There are no specific blogs for PIGZ, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PIGZ GST (N-Term) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol PIGZ