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Aldolase C Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Analysis in human cerebral cortex and pancreas tissues. Corresponding ALDOC RNA-seq data are presented for the same tissues.
Western Blot: Aldolase C Antibody [NBP1-90954] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Aldolase C Antibody [NBP1-90954] - Staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of glial cells.
Western Blot: Aldolase C Antibody [NBP1-90954] - Analysis in human cerebral cortex tissue.
Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Aldolase C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVI
Marker
pan-Astrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDOC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aldolase C Protein (NBP1-90954PEP)
Publications
Read Publication using
NBP1-90954 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25322816).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aldolase C Antibody

  • ALDC
  • aldolase 3
  • aldolase C, fructose-bisphosphate
  • Brain-type aldolase
  • EC 4.1.2.13
  • fructoaldolase C
  • fructose-1,6-biphosphate triosephosphate lyase
  • fructose-bisphosphate aldolase C

Background

The Aldolase C gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in thehippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversiblealdol cleavage of fructo

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aldolase C Antibody (NBP1-90954)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Aldolase C.

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.

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Bioinformatics

Gene Symbol ALDOC