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ALCAM/CD166 Antibody (105902) [Unconjugated]

 (11 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
105902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6561U-100UG
Alexa Fluor 405
FAB6561V-100UG
Alexa Fluor 488
FAB6561G
Alexa Fluor 532
FAB6561X
Alexa Fluor 594
FAB6561T-100UG
Alexa Fluor 647
FAB6561R-100UG
Alexa Fluor 700
FAB6561N-100UG
Alexa Fluor 750
FAB6561S-100UG
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FAB6561A
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FAB6561APCCY7
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BAM6561
CoraFluor 1
FAB6561CL1
DyLight 350
FAB6561D
DyLight 405
FAB6561E
DyLight 488
FAB6561K
DyLight 550
FAB6561L
DyLight 594
FAB6561M
DyLight 650
FAB6561W
DyLight 680
FAB6561Y
DyLight 755
FAB6561Z
FITC
FAB6561F
HRP
FAB6561H
Janelia Fluor 525
FAB6561JF525
Janelia Fluor 549
FAB6561I
Janelia Fluor 646
FAB6561J
Janelia Fluor 669
FAB6561JF669
PE/Atto594
FAB6561PEATT594
PE/Cy5.5
FAB6561PECY55
PE/Cy7
FAB6561PECY7
PerCP
FAB6561C
Phycoerythrin
FAB6561P
mFluor Violet 450 SE
FAB6561MFV450
mFluor Violet 500 SE
FAB6561MFV500
mFluor Violet 610 SE
FAB6561MFV610
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Carrier Free
FAB6561G
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MAB6561-SP
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MAB6561-500
Carrier Free
FAB6561T-100UG
Carrier Free
FAB6561S-100UG
Carrier Free
FAB6561R-100UG
Carrier Free
FAB6561N-100UG
Carrier Free
FAB6561V-100UG
Carrier Free
FAB6561U-100UG
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ALCAM/CD166 Antibody (105902) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Specificity
Detects human ALCAM/CD166 in Western blots. Shows approximately 50% cross‑reactivity with recombinant mouse OCAM and no cross‑reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALCAM
Purity Statement
Protein A or G purified from ascites
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human ALCAM/CD166 Biotinylated Antibody (Catalog number BAF656)
Standard: Recombinant Human ALCAM/CD166 Fc Chimera (Catalog number 656-AL)
Publications
Read Publications using
MAB6561 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody (105902) [Unconjugated]

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALCAM/CD166 Antibody (MAB6561)(11)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ELISA Development, Flow Cytometry, Western Blot.


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ELISA Development
(2)
Flow Cytometry
(5)
Western Blot
(1)
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Human
(7)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB6561 Applications Species
U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma Cancers (Basel), 2019-10-28;11(11):. 2019-10-28 [PMID: 31661833] (Flow Cytometry, Human) Flow Cytometry Human
SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019-02-01;0(0):. 2019-02-01 [PMID: 30720108] (Flow Cytometry, Rat) Flow Cytometry Rat
KE Hebron, EY Li, SA Arnold Egl, AK von Lersne, C Taylor, J Houkes, DK Flaherty, A Eskaros, TP Stricker, A Zijlstra Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis Sci Rep, 2018-02-16;8(1):3208. 2018-02-16 [PMID: 29453336] (Flow Cytometry, Human) Flow Cytometry Human
Maria Raffaella Zocchi, Caterina Camodeca, Elisa Nuti, Armando Rossello, Roberta Venè, Francesca Tosetti et al. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing OncoImmunology 2016-05-03 [PMID: 27467923]
Williams D, Anastos K, Morgello S, Berman J JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol, 2014-11-24;97(2):401-12. 2014-11-24 [PMID: 25420915] (Flow Cytometry, Human) Flow Cytometry Human
MIN YE, YAN-LEI DU, YU-QIANG NIE, ZHI-WEI ZHOU, JIE CAO, YING-FEI LI Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation Molecular Medicine Reports 2015-03-01 [PMID: 25395097]
Hansen A, Freeman T, Arnold S, Starchenko A, Jones-Paris C, Gilger M, Washington M, Fan K, Shyr Y, Beauchamp R, Zijlstra A Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome. Cancer Res, 2013-03-28;73(10):2955-64. 2013-03-28 [PMID: 23539446] (Western Blot, Human) Western Blot Human
Ihnen M, Kress K, Kersten JF, Kilic E, Choschzick M, Zander H, Muller V, Mahner S, Janicke F, Woelber L, Milde-Langosch K Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients. BMC Cancer, 2012-04-04;12(0):140. 2012-04-04 [PMID: 22475274] (ELISA Development, Human) ELISA Development Human
Marcio G. von Muhlen, Norman D. Brault, Scott M. Knudsen, Shaoyi Jiang, Scott R. Manalis Label-Free Biomarker Sensing in Undiluted Serum with Suspended Microchannel Resonators Analytical Chemistry 2010-03-01 [PMID: 20148583]
Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer. Int. J. Cancer, 2009-07-01;125(1):9-14. 2009-07-01 [PMID: 19322904] (ELISA Development, Human) ELISA Development Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol ALCAM
Uniprot