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AKT Antibody [Unconjugated] - Pan Specific

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and ...read more
Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 60 kDa (as indicated) using 20 µg/mL of Rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2055-SP
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AKT Antibody [Unconjugated] - Pan Specific Summary

Immunogen
Akt synthetic peptide
IRCLQWTTVIERTFHV
Accession # P31749
Specificity
Detects human Akt1, Akt2, and Akt3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using AF2055.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT Antibody [Unconjugated] - Pan Specific

  • RAC-ALPHA
  • AKT serine/threonine kinase 1
  • Akt
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC

Background

Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta , RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma , RAC-PK-gamma ).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKT Antibody (AF2055)(1)


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Product General Protocols

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Bioinformatics

Gene Symbol AKT1
Uniprot