![Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012] - Western blot analysis of AIP/ARA9 on different lysates with Rabbit anti-AIP/ARA9 antibody (NBP3-32012) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2:Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: THP-1 cell lysate Lane 5: HCT 1 cell lysate Lane 6: MCF7 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: Mouse spleen tissue lysate Lane 11: Rat spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissuel lysates/proteins at 40 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32012_rabbit-aip-ara9-mab-psh03-17-27820241514171.jpg "Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012] - Western blot analysis of AIP/ARA9 on different lysates with Rabbit anti-AIP/ARA9 antibody (NBP3-32012) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2:Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: THP-1 cell lysate Lane 5: HCT 1 cell lysate Lane 6: MCF7 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: Mouse spleen tissue lysate Lane 11: Rat spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissuel lysates/proteins at 40 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | PSH03-17 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within human AIP/ARA9 aa 1-330 / 330. (Uniprot: O00170) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | AIP |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for AIP/ARA9 Antibody (NBP3-32012)Find related products by research area.
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Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more... AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AIP |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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