AIF-1/Iba1 Antibody

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 9 of 9.

Publications using NB100-2833	Applications	Species
Awad-Igbaria Y, Ferreira N, Keadan A et al. HBO treatment enhances motor function and modulates pain development after sciatic nerve injury via protection the mitochondrial function Journal of Translational Medicine 2023-08-15 [PMID: 37582750]
Kawai M, Imaizumi K, Ishikawa M Et al. Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor function Cell reports 2021-11-23 [PMID: 34818559] (IHC-P, Mouse)	IHC-P	Mouse
Guyot AC, Leuxe C, Disdier C et al. A Small Compound Targeting Prohibitin with Potential Interest for Cognitive Deficit Rescue in Aging mice and Tau Pathology Treatment Sci Rep 2020-01-24 [PMID: 31980673] (IHC-Fr)	IHC-Fr
Velazquez R, Ferreira E et al. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation. Aging Cell 2019-01-12 [PMID: 31560162] (IF/IHC, Mouse)	IF/IHC	Mouse
Neckles VN, Morton MC, Holmberg JC et al. A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles Sci Rep 2019-02-28 [PMID: 30816224] (IF/IHC, Mouse)	IF/IHC	Mouse
Ilievski V, Zuchowska Pk, Green SJ et al. Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice. PLoS ONE. 2018-10-03 [PMID: 30281647] (IF/IHC, Mouse)	IF/IHC	Mouse
Morton MC, Neckles VN, Seluzicki CM et al. Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen. Cell Rep 2018-04-03 [PMID: 29617675] (IF/IHC, Mouse)	IF/IHC	Mouse
Arsenault D, Coulombe K, Zhu A et al. Loss of Metabotropic Glutamate Receptor 5 Function on Peripheral Benzodiazepine Receptor in Mice Prenatally Exposed to LPS. PLoS ONE. 2015-11-05 [PMID: 26536027] (WB, Mouse)	WB	Mouse
Yang ZF, Ho DW, Lau CK et al. Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages. Int Immunol 2005-11-01 [PMID: 16157606]

Array NB100-2833

• AIF-1/Iba1 Antibodies
• AIF-1/Iba1 ELISA Kits
• AIF-1/Iba1 Lysate
• AIF-1/Iba1 Proteins