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AGO1/EIF2C1 Antibody

Images

 
Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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AGO1/EIF2C1 Antibody Summary

Immunogen
Peptide with sequence C-KNASYNLDPYIQEF corresponding to internal region according to NP_036331.1.
Specificity
This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32812257)WB: In transfected HEK293 transiently expressing human EIF2C1bands of approx. 110 Knockdown Validateda band and 35 Knockdown Validateda band are observed. The 110 Knockdown Validateda band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 Knockdown Validateda band according to NP_036331.1
Control
AGO1/EIF2C1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-2817 in the following applications:

Publications
Read Publications using
NB100-2817 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AGO1/EIF2C1 Antibody

  • AGO1Q99
  • argonaute 1
  • argonaute1
  • DKFZp686M13167
  • eIF2C 1
  • eIF-2C 1
  • EIF2C
  • Eukaryotic translation initiation factor 2C 1
  • eukaryotic translation initiation factor 2C, 1
  • GERP95
  • Golgi Endoplasmic Reticulum protein 95 kDa
  • hAgo1
  • protein argonaute-1
  • Putative RNA-binding protein Q99

Background

The AGO1/EIF2C1 gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-me

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AGO1/EIF2C1 Antibody (NB100-2817)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, WB, Western Blot.


Filter By Application
ICC/IF
(1)
WB
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
All Species

Review for AGO1/EIF2C1 Antibody (NB100-2817) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2817:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Souvik Ghosh
WB Human 05/07/2021
View

Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
LotNo Record

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AGO1/EIF2C1 Antibody (NB100-2817) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for AGO1/EIF2C1 Antibody (NB100-2817)

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Blogs on AGO1/EIF2C1

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Souvik Ghosh
05/07/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol AGO1
Entrez
Uniprot