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![Western Blot: AF9 Antibody [NBP2-15303] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NBP2-15303-img0009.jpg "Western Blot: AF9 Antibody [NBP2-15303] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15303-img0004.jpg)
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse liver. AF9 stained by AF9 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: AF9 Antibody [NBP2-15303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NBP2-15303-img0006.jpg)
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - D54 xenograft, using AF9 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0002.jpg)
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse brain. AF9 stained by AF9 antibody diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0007.jpg)
![Western Blot: AF9 Antibody [NBP2-15303] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (NBP2-15303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15303_rabbit-polyclonal-af9-antibody-91020241957219.jpg)
![Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1566 and another AF9 Ab.](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NB100-1565-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1566 and another AF9 Ab.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-NB100-1565-img0003.jpg)
![Western Blot: AF9 Antibody [NBP1-90217] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MLLT3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NBP1-90217-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AF9 Antibody [NBP1-90217] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MLLT3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: AF9 Antibody [NBP1-90217] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NBP1-90217-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AF9 Antibody [NBP1-90217] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90217-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - AF9 antibody [HL1644] detects AF9 protein at nucleus and granule by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 stained by AF9 antibody [HL1644] (NBP3-25354) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25354_rabbit-af9-mab-hl1644-azide-and-bsa-free-2120249413393.jpg "Immunocytochemistry/Immunofluorescence: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - AF9 antibody [HL1644] detects AF9 protein at nucleus and granule by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 stained by AF9 antibody [HL1644] (NBP3-25354) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (NBP3-25354) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25354_rabbit-af9-mab-hl1644-azide-and-bsa-free-2120249362737.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (NBP3-25354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25354_rabbit-af9-mab-hl1644-azide-and-bsa-free-2120249413346.jpg)
![Western Blot: AF9 Overexpression Lysate (Adult Normal) [NBL1-13137] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AF9.](http://images.novusbio.com/fullsize/AF9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13137-img0002.jpg "Western Blot: AF9 Overexpression Lysate (Adult Normal) [NBL1-13137] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AF9.")
![ELISA: AF9 Antibody (3C11) [H00004300-M08] - Detection limit for recombinant GST tagged MLLT3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AF9-Antibody-3C11-ELISA-H00004300-M08-img0001.jpg "ELISA: AF9 Antibody (3C11) [H00004300-M08] - Detection limit for recombinant GST tagged MLLT3 is approximately 3ng/ml as a capture antibody.")