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ADP-Sugar Pyrophosphatase/NUDT5 Antibody

Images

 
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ADP-Sugar Pyrophosphatase/NUDT5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLKF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUDT5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ADP-Sugar Pyrophosphatase/NUDT5 Protein (NBP1-83131PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ADP-Sugar Pyrophosphatase/NUDT5 Antibody

  • EC 3.6.1.-
  • EC 3.6.1.13
  • hYSAH1
  • nucleoside diphosphate linked moiety X-type motif 5
  • Nucleoside diphosphate-linked moiety X motif 5
  • nudix (nucleoside diphosphate linked moiety X)-type motif 5
  • Nudix motif 5
  • NUDT5
  • YSA1
  • YSA1H
  • YSA1HADP-sugar pyrophosphatase

Background

Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan, 1999 [PubMed 10373642]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NUDT5