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ADO Antibody

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Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human liver shows low positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human testis shows cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human cerebral cortex shows strong positivity in neurons.
Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human smooth muscle shows moderate positivity.
Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human liver shows no positivity in hepatocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Analysis in human testis and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] - Analysis in human testis and liver tissues. Corresponding ADO RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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ADO Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GASDRDAASGPEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ADO Protein (NBP1-88530PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ADO Antibody

  • 2-aminoethanethiol (cysteamine) dioxygenase
  • 2-aminoethanethiol dioxygenase
  • C10orf22
  • chromosome 10 open reading frame 22
  • cysteamine (2-aminoethanethiol) dioxygenase (ADO)
  • Cysteamine dioxygenase
  • DKFZp564C046
  • EC 1.13.11.19
  • FLJ14547

Background

Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase(ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine toform hypotaurine (Dominy et al., 2007 (PubMed 17581819)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ADO