![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Ig2838-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09003-img0001.jpg "Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF, B/N |
| Clone | Ig2838 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Human recombinant A2-adenosine receptor. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ADORA2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to the A2 adenosine receptor, a G-protein-coupled receptor for adenosine that, upon ligand binding, activates adenylyl cyclase. The antibody acts as an allosteric inhibitor. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Adenosine A2aR Antibody (NBP3-09003)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADORA2A |
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")