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ADAM15 Antibody [Unconjugated]

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ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF935
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF935-SP
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ADAM15 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Ectodomain
Leu18-Ser693
Accession # Q13444
Specificity
Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADAM15
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF935 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM15 Antibody [Unconjugated]

  • ADAM 15
  • ADAM metallopeptidase domain 15
  • ADAM15
  • disintegrin-like, and cysteine-rich protein 15
  • EC 3.4.24
  • MDC15
  • MDC-15
  • Metargidin

Background

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM15 Antibody
[Unconjugated]
AF935
ADAM15 Antibody [Unconjugated]
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Publications for ADAM15 Antibody (AF935)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: IF, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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IF
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(2)
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Human
(4)
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Product General Protocols

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Bioinformatics

Gene Symbol ADAM15
Uniprot