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Activin B/Inhibin beta B Antibody (146807)

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Recombinant Human Activin B beta B subunit (Catalog # 659-AB) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Human Activin B beta B subunit (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Activin B beta B subunit Monoclonal Antibody (Catalog # MAB659). The ND50 is typically 0.6-3.0 µg/mL.
Recombinant Human Activin B beta B subunit (Catalog # 659-AB) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
146807
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB659-SP
Carrier Free
MAB659-500
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Activin B/Inhibin beta B Antibody (146807) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Activin B
Gly293-Ala407
Accession # Q53T31
Specificity
Detects human Activin B beta B subunit in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Inhibin B or rhActivin A is observed. In Western blots, approximately 20% cross-reactivity with rhInhibin B is observed and no cross‑reactivity with rhActivin A or recombinant mouse Activin A is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
INHBB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.6-3.0 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB659 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin B/Inhibin beta B Antibody (146807)

  • activin AB beta polypeptide
  • Activin Beta B
  • Activin beta-B chain
  • INHBB
  • inhibin beta B chain
  • Inhibin Beta B
  • Inhibin Subunit Beta B
  • inhibin, beta B (activin AB beta polypeptide)
  • inhibin, beta B
  • Inhibin, beta-2

Background

Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha  subunit and one of the various beta  subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and  Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A­‑ beta A), activin B ( beta B­‑ beta B), activin AB ( beta A‑ beta B), inhibin A ( alpha ­‑ beta A) and inhibin B ( alpha ­‑ beta B). At present, little is known about the contribution of the other beta  subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha  subunits share approximately 80% identity. Similarly to other TGF-beta  family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low-affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.

  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Activin B/Inhibin
beta B Antibody (1468 ...
MAB659
Activin B/Inhibin beta B Antibody (146807)
Species: Hu
Applications: WB, B/N

Publications for Activin B/Inhibin beta B Antibody (MAB659)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-P, Neutralization, Western Blot.


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Flow Cytometry
(1)
IHC
(1)
IHC-P
(1)
Neutralization
(2)
Western Blot
(1)
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Human
(3)
Mouse
(1)
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(1)
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Showing Publications 1 - 5 of 5.
Publications using MAB659 Applications Species
KL Reader, S John-McHaf, S Zellhuber-, T Jowett, DG Mottershea, HE Cunliffe, EJ Gold Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth Cancers, 2022-12-27;15(1):. 2022-12-27 [PMID: 36612143] (IHC, Human) IHC Human
M Morabito, M Larcher, FM Cavalli, C Foray, A Forget, L Mirabal-Or, M Andrianter, S Druillenne, A Garancher, J Masliah-Pl, S Leboucher, A Debalkew, A Raso, O Delattre, S Puget, F Doz, MD Taylor, O Ayrault, F Bourdeaut, A Eychène, C Pouponnot An autocrine ActivinB mechanism drives TGF?/Activin signaling in Group 3 medulloblastoma EMBO Mol Med, 2019-07-22;0(0):e9830. 2019-07-22 [PMID: 31328883] (Neutralization, Human) Neutralization Human
N Spottiswoo, AE Armitage, AR Williams, AJ Fyfe, S Biswas, SH Hodgson, D Llewellyn, P Choudhary, SJ Draper, P Duffy, H Drakesmith The role of activins in hepcidin regulation during malaria Infect. Immun., 2017-11-17;0(0):. 11/17/2017 [PMID: 28893916]
Dussiot M, Maciel T, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil J, Arlet J, Cote F, Courtois G, Ginzburg Y, Daniel T, Chopra R, Sung V, Hermine O, Moura I An activin receptor IIA ligand trap corrects ineffective erythropoiesis in beta-thalassemia. Nat Med, 2014-03-23;20(4):398-407. 2014-03-23 [PMID: 24658077] (Flow Cytometry, IHC-P, Neutralization, Mouse) Flow Cytometry, IHC-P, Neutralization Mouse
Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases. J. Neurochem., 2007-08-06;103(3):962-71. 2007-08-06 [PMID: 17680997] (Western Blot, Human, Rat) Western Blot Human, Rat

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Bioinformatics

Gene Symbol INHBB
Uniprot