![Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Western-Blot-NBP2-80561-img0003.jpg "Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, ZeSpecies Glossary |
| Applications | WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | mAbGEa |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46] |
| Localization | Cytoplasm > cytoskeleton |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTA1 |
| Purity | IgM purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, a band is seen at ~45 kDa representing Actin. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | IgM purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Actin Antibody (NBP2-80561)Find related products by research area.
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Migrasomes: A Novel Vesicle Involved in Intercellular Signaling By Christina Towers, PhD What are migrasomes?A novel vesicular organelle was recently discovered in 2015 by a research group at Tsinghua University headed by Dr. Yu that is dependent on cell migration... Read full blog post. |
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Considerations for Quantitative Western blotting By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell... Read full blog post. |
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Repurposing FDA-approved drugs to combat the rise of antibiotic resistance By Beth Melson, MSAntibiotic resistance is a global threat to public health. Widespread, inappropriate use of antibiotics, such as to treat viral infections or promote growth in livestock, has led to increased incid... Read full blog post. |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation. Just like any other model organism, it is important to have an effective loading control to va... Read full blog post. |
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CRISPR/Cas9: Keep your friends close, but your viruses closer "CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism. Specifically, the CRISPR sequence has been identified as a si... Read full blog post. |
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Alpha-actin/ACTA1 - A skeletal muscle isoform mutated in various myopathies Actin is an abundant cytoskeletal protein involved in a variety of cellular processes such as cell motility, cell division, and muscle contraction. Actin monomers assemble into filaments and can provide a track for transport of cargo by the molecul... Read full blog post. |
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Alpha-Adducin - Assembling the cytoskeleton meshwork that underlies the plasma membrane The structure and organization of the plasma membrane is maintained by an underlying network of cytoskeletal proteins including actin and spectrin. Adducin, a member of this protein network, binds to bundles and caps actin filaments and links them... Read full blog post. |
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Understanding Actin Alpha 2 Smooth Muscle Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated... Read full blog post. |
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Could Laminin be Used to Treat Duchenne Muscular Dystrophy? Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or... Read full blog post. |
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| Gene Symbol | ACTA1 |
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80561-img0007.jpg "Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80561-img0011.jpg "Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues. Image from the standard format of this antibody.")
![Flow Cytometry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80561-img0012.jpg "Flow Cytometry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue). Image from the standard format of this antibody.")
![Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Western-Blot-NBP2-80561-img0001.jpg "Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates. Image from the standard format of this antibody.")
![Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Western-Blot-NBP2-80561-img0002.jpg "Western Blot: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80561-img0004.jpg "Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 2 ug/mL overnight at 4C and detected with an anti-mouse IgM DyLight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80561-img0005.jpg "Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 2 ug/mL overnight at 4C and detected with an anti-mouse IgM DyLight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80561-img0006.jpg "Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80561-img0008.jpg "Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80561-img0009.jpg "Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. Image from the standard format of this antibody.")
![Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80561-img0010.jpg "Immunohistochemistry: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues. Image from the standard format of this antibody.")
![Simple Western: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Azide-and-BSA-Free-Simple-Western-NBP2-80561-img0013.jpg "Simple Western: Actin Antibody (mAbGEa) - Azide and BSA Free [NBP2-80561] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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