Actin Alpha 1 Cardiac Muscle Antibody (959606) [Unconjugated] Summary
Immunogen |
E.coli-derived recombinant human alpha -Cardiac Actin Asp3-Phe377 Accession # P68032 |
Specificity |
Detects human alpha -Cardiac Actin in direct ELISAs and Western blots.
|
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 5-25 ug/mL
- Immunohistochemistry 1-25 ug/mL
- Simple Western 2 ug/mL
- Western Blot 0.1 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (959606) [Unconjugated]
Background
Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal
filament in cardiac muscle which interacts with the 50-KDa domain of the myosin
motor domain in cardiac muscle contraction. Actin is highly conserved across
species, and differs from skeletal muscle actin (ACTA1) by only four amino
acids. Mutations in the ACTC1 gene are linked to familial hypertrophy
cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory
cardiomyopathy.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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