Novus Biologicals products are now on bio-techne.com

ACAT1 Recombinant Protein Antigen

Images

 
There are currently no images for ACAT1 Protein (NBP1-89285PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

ACAT1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ACAT1.

Source: E. coli

Amino Acid Sequence: VSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTD

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
ACAT1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-89285.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for ACAT1 Recombinant Protein Antigen

  • ACAT
  • ACAT1
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Background

Sterol O-acyltransferase 1 (SOAT1), also known as ACAT1, catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys. Specifically, ACAT-1 catalyzes the reversible reaction forming acetoacetyl-CoA from two molecules of acetyl-CoA. Additionaly, the ACAT 1 protein may have a role in lipoprotein assembly and dietary cholesterol absorption, and may also act as a ligase.

Mutations in this gene have been associated with 3-ketothiolase deficiency, an inborn defect of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-92630
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
DKK300
Species: Hu
Applications: ELISA
NBP2-45981
Species: Hu
Applications: IHC, IHC-P
NB100-64852
Species: Hu
Applications: Flow, IF, IHC, IHC-Fr
AF4036
Species: Hu
Applications: Simple Western, WB
H00005324-M02
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
M6000B
Species: Mu
Applications: ELISA
NBP2-93808
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
7770-GT
Species: Hu
Applications: EnzAct
NBP2-66888
Species: Hu, Mu, Rt
Applications: IHC-P, IP, Simple Western, WB
NB100-92243
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PLA, WB
NB400-141
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
7954-GM/CF
Species: Hu
Applications: BA
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP2-01863
Species: Hu, Mu
Applications: IHC, IHC-P, WB

Publications for ACAT1 Protein (NBP1-89285PEP) (0)

There are no publications for ACAT1 Protein (NBP1-89285PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ACAT1 Protein (NBP1-89285PEP) (0)

There are no reviews for ACAT1 Protein (NBP1-89285PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ACAT1 Protein (NBP1-89285PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ACAT1 Products

Research Areas for ACAT1 Protein (NBP1-89285PEP)

Find related products by research area.

Blogs on ACAT1

There are no specific blogs for ACAT1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

ACAT1 Antibody
NBP1-89284

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our ACAT1 Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol ACAT1