ACAT1 Antibodies
We offer ACAT1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each ACAT1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ACAT1 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Rat. Use the list below to choose the ACAT1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ACAT1 polyclonal antibodies and browse our ACAT1 monoclonal antibody catalog.
Alternate Names for ACAT1 Antibodies
anti-ACAT1 antibody, anti-ACAT antibody, anti-acetoacetyl Coenzyme A thiolase antibody, anti-Acetoacetyl-CoA thiolase antibody, anti-acetyl-CoA acetyltransferase 1 antibody, anti-acetyl-CoA acetyltransferase, mitochondrial antibody, anti-acetyl-Coenzyme A acetyltransferase 1 antibody, anti-EC 2.3.1 antibody, anti-EC 2.3.1.9 antibody, anti-MAT antibody, anti-mitochondrial acetoacetyl-CoA thiolase antibody, anti-T2 antibody, anti-THIL antibody
6 Results for "acat1" in Primary Antibodies
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183319.jpg "Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183320.jpg)
![Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183336.jpg)
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183337.jpg)
![Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0003.jpg "Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody (15E5) [NBP2-22565] - Analysis of ACAT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Immunocytochemistry-Immunofluorescence-NBP2-22565-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0001.jpg)
![Western Blot: ACAT1 Antibody (S02-5G8) [NBP3-19555] - Western blot detection of ACAT1 in K562, Hela cell lysates using NBP3-19555 (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.](http://images.novusbio.com/fullsize/ACAT1-Antibody-S02-5G8-Western-Blot-NBP3-19555-img0001.jpg "Western Blot: ACAT1 Antibody (S02-5G8) [NBP3-19555] - Western blot detection of ACAT1 in K562, Hela cell lysates using NBP3-19555 (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.")
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar protein distribution across tissues to independent antibody NBP1-89284 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar protein distribution across tissues to independent antibody NBP1-89284 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar pattern to independent antibody NBP1-89284 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89285] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89285-img0009.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0016.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum shows strong positivity in mitochondria in purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0013.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0014.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89285] - ACAT1 protein expression in wild-type and acat-1-/- macrophages. Cell lysates were immunoblotted and ACAT1 protein levels were compared with GAPDH as loading control. CRISPR/Cas-9 used to knockout acat-1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29390006/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Knockout-Validated-NBP1-89285-img0017.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89285] - Blocking LD formation increases C. burnetii growth.Wild-type C. burnetii growth in infected MH-S cells treated with different inhibitors was measured at 2 & 4 days post-infection by FFU assay. A-D) Representative images for wild-type MH-S macrophages treated with inhibitors, fixed, stained for PLIN2 (LDs; green) & C. burnetii (red) & imaged day 4 post-treatment at 100X. Scale bar = 10 µm. E) Growth while inhibiting LD formation with triacsin C (10 µM) in wild-type MH-S macrophages. Error bars represent the mean of 4 independent experiments +/- SEM. ** = p <0.01 compared to vehicle-treated cells as determined by two-way ANOVA with Bonferroni post-hoc test. F) ACAT1 protein expression in wild-type & acat-1-/- macrophages. Cell lysates were immunoblotted & ACAT1 protein levels were compared with GAPDH as loading control. G) C. burnetii growth in vehicle-treated wild-type & acat-1-/- MH-S macrophages & (H) T863-treated acat-1-/- MH-S macrophages. Error bars represent the mean of at least 3 independent experiments +/- SEM., * = p<0.05, *** = p <0.001 as determined by two-way ANOVA with Bonferroni post-hoc test. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0192215), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89285_rabbit-polyclonal-acat1-antibody-310202416121928.jpg)