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ABH2 Antibody

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Immunocytochemistry/ Immunofluorescence: ABH2 Antibody [NBP2-55544] - Staining of human cell line HEK 293 shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ABH2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALKBH2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ABH2 Recombinant Protein Antigen (NBP2-55544PEP)

Reactivity Notes

Mouse 89%, Rat 88%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ABH2 Antibody

  • 2OG-Fe(II) oxy DC1
  • ABH2FLJ99103
  • alkB, alkylation repair homolog 2 (E. coli)
  • Alkylated DNA repair protein alkB homolog 2
  • alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
  • EC 1.14.11.-
  • MGC90512
  • Oxy DC1

Background

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNAlesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze theremoval of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 (PubMed 12486230)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALKBH2