![Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - DEPC-1 monoclonal antibody (M01), clone 2A5-4F5 Analysis of DEPC-1 expression in K-562.](http://images.novusbio.com/fullsize/ALKBH3-Antibody-2A5-4F5-Western-Blot-H00221120-M01-img0004.jpg "Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - DEPC-1 monoclonal antibody (M01), clone 2A5-4F5 Analysis of DEPC-1 expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 2A5-4F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | DEPC-1 (AAH15155, 1 a.a. ~ 139 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW |
| Specificity | DEPC-1 - prostate cancer antigen-1 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ALKBH3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Sandwich ELISA: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - Detection limit for recombinant GST tagged DEPC-1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ALKBH3-Antibody-2A5-4F5-Sandwich-ELISA-H00221120-M01-img0003.jpg "Sandwich ELISA: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - Detection limit for recombinant GST tagged DEPC-1 is approximately 0.03ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")