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ABCB5 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: ABCB5 Antibody [NBP1-77687] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: ABCB5 Antibody [NBP1-77687] - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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ABCB5 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human ABCB5 protein (between residues 150-200) [UniProt Q2M3G0]
Localization
Cell membrane; Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCB5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot reported in scientific literature (PMID 25030174)
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
A-431 Whole Cell Lysate
Publications
Read Publications using
NBP1-77687 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCB5 Antibody - BSA Free

  • ABCB5 P-gp
  • ABCB5
  • ABCB5alpha
  • ABCB5beta
  • ATP-binding cassette protein
  • ATP-binding cassette sub-family B member 5
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • EC 3.6.3
  • EC 3.6.3.44
  • EST422562
  • P-glycoprotein ABCB5

Background

The ATP-binding cassette sub-family B member 5 (ABCB5) is an ABC transporter and PGP member. It is highly expressed in skin and in malignant melanoma. When two abnormal growths of tissues occur, resulting in multiple malignancies, ABCB5 is often highly expressed. This elevated expression in tumor cells can indicate a propensity towards therapeutic failure in many cases. Analysis of ABCB5 concentrations in tumors has been suggested as a fairly dependable qualitative biomarker for assessing the malignancy of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCB5 Antibody (NBP1-77687)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(1)
WB
(1)
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Human
(2)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCB5 Antibody (NBP1-77687) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ABCB5 Products

Array NBP1-77687

Research Areas for ABCB5 Antibody (NBP1-77687)

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Blogs on ABCB5

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Bioinformatics

Gene Symbol ABCB5
Entrez
Uniprot