A-431 Whole Cell Lysate Summary
| Specificity |
A431 whole cell extract lysate |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8] |
| Concentration |
1 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Skin |
| Subcellular Fraction |
Whole |
Background
This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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![Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.](http://images.novusbio.com/fullsize/A-431-Whole-Cell-Lysate-Western-Blot-NB800-PC12-img0002.jpg "Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.")
![Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stain of 0.02 ml of NB 800-PC12 separated in a 4-20% gradient gel under non-reducing conditions (lane 1). MW standard is shown in lane 2.](http://images.novusbio.com/fullsize/A-431-Whole-Cell-Lysate-Western-Blot-NB800-PC12-img0001.jpg "Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stain of 0.02 ml of NB 800-PC12 separated in a 4-20% gradient gel under non-reducing conditions (lane 1). MW standard is shown in lane 2.")