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A33/GPA33 Antibody [Unconjugated]

 
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Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) ...read more
TH‑29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (Catalog # ...read more
A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for A33 at approximately 64 kDa (as indicated) using 20 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3080
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3080-SP
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A33/GPA33 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Specificity
Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPA33
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A33/GPA33 Antibody [Unconjugated]

  • A33
  • cell surface A33 antigen
  • glycoprotein A33 (transmembrane)
  • Glycoprotein A33
  • GPA33
  • MGC129986
  • MGC129987
  • transmembrane glycoprotein A33

Background

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2, and JAM-A, -B, and -C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD shares 80%, 74%, and 71% aa sequence identity with canine, bovine, and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GPA33
Uniprot