![Western Blot: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Western-Blot-NBP2-70166-img0002.jpg "Western Blot: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI3F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 18-364 of human ALOX5(NP_000689) produced in E.coli. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ALOX5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 77.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for 5-Lipoxygenase Antibody (NBP2-70166)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ALOX5 |
![Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70166-img0006.jpg "Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
![Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70166-img0003.jpg "Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)")
![Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70166-img0004.jpg "Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).")
![Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70166-img0005.jpg "Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)")
![Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-OTI3F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70166-img0007.jpg "Immunohistochemistry: 5-Lipoxygenase Antibody (OTI3F1) - Azide and BSA Free [NBP2-70166] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).")
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