![Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-Western-Blot-NBP1-06976-img0004.jpg "Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-HYKTDVAVKDDPE corresponding to internal region according to NP_001131.3. |
| Epitope | internal region |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ALOX15 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3).Primary incubation was 1 hour. IHC: Paraffin embedded Human Skeletal Muscle and Kidney. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-06976 | Applications | Species |
|---|---|---|
| McDuffie M, Maybee NA, Keller SR et al. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes 2008-01-01 [PMID: 17940120] |
Secondary Antibodies |
Isotype Controls |
Research Areas for 15-Lipoxygenase 1 Antibody (NBP1-06976)Find related products by research area.
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| Gene Symbol | ALOX15 |
| Entrez |
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| Uniprot |
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![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-06976-img0003.jpg "Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-06976-img0002.jpg "Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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