| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77681SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77253-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4257-SP | |||
| Carrier Free | IC4257V-100UG | |||
| Carrier Free | IC4257U-100UG | |||
| Carrier Free | IC4257T-100UG | |||
| Carrier Free | IC4257S-100UG | |||
| Carrier Free | IC4257R-100UG | |||
| Carrier Free | IC4257N-100UG | |||
| Carrier Free | IC4257G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77252-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75514-0.025mg | |||
![Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77681-img0006.jpg "Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0010.jpg)
![Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-NBP1-77681-img0004.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0009.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0007.jpg)
![Western Blot: XBP1 Antibody [NBP1-77681] - Western Blot of PC-3 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-77681_rabbit-polyclonal-xbp1-antibody-western-blot-672023151929..jpg)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414593.jpg "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: XBP1 Antibody - BSA Free [NBP1-77253] - KO Validation in 293T Cells. Loading: 10 u of lysate Antibodies: XBP-1, 4 u/mL and beta-actin, 1u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202419313294.gif)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg)
![Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - Validation in Mouse Pancreas Tissue.Loading: 15 u of lysates per lane.Antibodies: XBP-1, (4 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241918647.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Mouse Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241956210.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Liver Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420312941.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120242013185.gif)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420441134.jpg)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414538.jpg)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Rat Spleen Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241923693.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0004.jpg "Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77252-img0002.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252] - Immunocytochemistry of XBP1 in HepG2 cells with XBP1 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-77252_rabbit-polyclonal-xbp1-antibody-271120241918381.jpg)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252] - Immunocytochemistry of XBP1 in HepG2 cells with XBP1 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-77252_rabbit-polyclonal-xbp1-antibody-271120242045638.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Western Blot: XBP1 Antibody [NBP2-20917] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0001.jpg)
![Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Western-Blot-NBP1-75514-img0001.jpg "Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] - Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 4 u/mL.](http://images.novusbio.com/fullsize/nbp1-75514_mouse-monoclonal-xbp1-antibody-3h1g4-2711202419353897.jpg)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] - Immunocytochemistry of XBP1 in HepG2 cells with XBP1 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-75514_mouse-monoclonal-xbp1-antibody-3h1g4-271120241918648.jpg)
![Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.](http://images.novusbio.com/fullsize/XBP1-Antibody-S04-3E2-Western-Blot-NBP3-19927-img0001.jpg "Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBL1-17900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17900-img0002.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBL1-17900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-08472] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08472-img0001.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-08472] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-09267] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09267-img0001.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-09267] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![ELISA: Human XBP1 ELISA Kit (Colorimetric) [NBP3-38912] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38912_human-xbp1-elisa-kit-colorimetric-2110202415362323.png "ELISA: Human XBP1 ELISA Kit (Colorimetric) [NBP3-38912] - Standard Curve Reference")
![ELISA: Human XBP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38911] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38911_human-xbp1-ready-to-use-elisa-kit-colorimetric-2110202415334948.png "ELISA: Human XBP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38911] - Standard Curve Reference")