![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414593.jpg "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP1. The immunogen is located within amino acids 40 - 90 of XBP1. Amino Acid Sequence: GLPQARKRQRLTHLSPE |
| Specificity | Human XBP1 has two isoforms, including isoform 1 (261aa, 29kD) and isoform 2 (376aa, 40kD). Mouse XBP1 also has two isoforms, including isoform 1 (267aa, 29.6kD) and isoform 2 (371aa, 40kD). And Rat XBP1 has two isoforms as well, including isoform 1 (267aa, 30kD) and isoform 2 (371aa, 40kD). NBP1-77253 can detect human, mouse and rat. |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | XBP1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 28, 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry-Paraffin IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Immunohistochemistry-Paraffin-NB100-2323-img0025.jpg)
![Western Blot IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NB100-2323-img0027.jpg)
![Western Blot IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NB100-2323-img0029.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 09/19/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for XBP1 Antibody (NBP1-77253)Find related products by research area.
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IRE1 alpha Regulates Dendritic Cell Function in Response to Viral Infection By Natalia Gurule, PhD What is IRE1 alpha?Inositol- requiring enzyme type 1 alpha (IRE1a) is a serine/threonine kinase that is one of the three transducers within the unfolded protein response (UPR) signalin... Read full blog post. |
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Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U... Read full blog post. |
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IRE1 - an important sensor in the unfolded protein response pathway During cellular stress the protein folding capacity of the ER is diminished. In order to maintain homeostasis and ensure proper protein folding cells activate the unfolded protein response (UPR), a signaling network consisting of sensors and effect... Read full blog post. |
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![Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - KO Validation in 293T Cells. Loading: 10 u of lysate Antibodies: XBP-1, 4 u/mL and beta-actin, 1u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202419313294.gif "Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - KO Validation in 293T Cells. Loading: 10 u of lysate Antibodies: XBP-1, 4 u/mL and beta-actin, 1u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p")
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL")
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL")
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at")
![Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - Validation in Mouse Pancreas Tissue.Loading: 15 u of lysates per lane.Antibodies: XBP-1, (4 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241918647.gif "Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - Validation in Mouse Pancreas Tissue.Loading: 15 u of lysates per lane.Antibodies: XBP-1, (4 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Mouse Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241956210.gif "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Mouse Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Liver Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420312941.gif "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Liver Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120242013185.gif "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420441134.jpg "Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414538.jpg "Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Rat Spleen Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241923693.jpg "Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Rat Spleen Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry BMP-3b/GDF-10 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1543_human-bmp-3b-gdf-10-affinity-purified-polyclonal-ab-immunocytochemistry-133202414504.jpg)
![Western Blot PERK Antibody [Unconjugated]](https://images.novusbio.com/images2/PERK_AF3999_Western_Blot_5379.jpg)
![Knockout Validated PERK Antibody [Unconjugated]](https://images.novusbio.com/images2/PERK_AF3999_Knockout_Validated_22482.jpg)
![Western Blot PERK Antibody [Unconjugated]](https://images.novusbio.com/images/af3999_human-perk-affinity-purified-polyclonal-ab-4120241240258.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg)
![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
