XBP1 Antibody (525904) - (S Isoform) Summary
Immunogen |
E. coli-derived recombinant human XBP1S Ser188-Leu287 Accession # NP_001073007 |
Specificity |
Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced). |
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
XBP1 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
|
Application Notes |
In Simple Western only 10-15 uL of the recommended dilution is used per data point. |
Reviewed Applications |
Read 1 Review rated 5 using MAB4257 in the following applications:
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Publications |
Read Publications using MAB4257 in the following applications:
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for XBP1 Antibody (525904) - (S Isoform)
Background
X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for XBP1 Antibody (MAB4257) (0)