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XBP1 Antibody (525904) - (S Isoform)

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RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
525904
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Support & Research

XBP1 Antibody (525904) - (S Isoform) Summary

Immunogen
E. coli-derived recombinant human XBP1S
Ser188-Leu287
Accession # NP_001073007
Specificity
Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XBP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
MAB4257 in the following applications:

Publications
Read Publications using
MAB4257 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XBP1 Antibody (525904) - (S Isoform)

  • HTF
  • TREB5
  • X-box binding protein 1
  • X-box-binding protein 1
  • XBP1
  • XBP-1
  • XBP2
  • XBP2Tax-responsive element-binding protein 5

Background

X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

5
5
1
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0
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1
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Natalie Erdmann
08/17/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol XBP1
Uniprot