| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 210-TA-02M/CF | |||
| Carrier Free | 210-TA-020/CF | |||
| Carrier Free | 210-TA-100/CF | |||
| Carrier Free | 210-TA-005/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 410-MT-010/CF | |||
| Carrier Free | 410-MT-050/CF | |||
| Carrier Free | 410-MT-025/CF | |||
| Carrier Free | 410-MT-100/CF | |||
| Carrier Free | 410-MT-01M/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-410-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19532SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB610-SP | |||
| Carrier Free | MAB610-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 510-RT-010/CF | |||
| Carrier Free | 510-RT-050/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
![N/A TNF-alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_DY410_ELISA_2323.jpg "N/A TNF-alpha [Biotin]")
![N/A TNF-alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_DY210_ELISA_1766.jpg "N/A TNF-alpha [Biotin]")
![N/A TNF-alpha [HRP]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_RTA00_ELISA_1099.jpg "N/A TNF-alpha [HRP]")
![N/A TNF-alpha [HRP]](http://images.novusbio.com/fullsize2/TNF-alpha_PRTA00_ELISA_365.jpg)
![WNT-5A induces a proinflammatory transformation in mouse microglia. (A, B) iNOS, COX-2 and TNF alpha were detected by immunoblotting in lysates from mouse primary microglia after WNT-5A stimulation (ctrl, 300 ng/ml, 6 hours). (B’) shows TNF alpha levels in the supernatant of primary microglia under ctrl conditions and upon WNT-5A stimulation (ctrl, 300 ng/ml, 24 hours; n = 4). At least three experiments are summarized in the bar graphs. Data are normalized to ctrl. Error bars give s.e.m. (C) Microglial proliferation was assessed by an MTT assay monitoring mitochondrial activity, which is proportional to cell number [see Additional file 1: Figure S5]. Stimulation with WNT-5A (300 ng/ml, 24 hours) increased MTT, which was blocked by PTX (100 ng/ml, overnight) or the MEK1/2 (10 μM) inhibitor, SL327. (D). Experiments were done in triplicate and data from three independent experiments are shown. *, P < 0.01; ***, P < 0.001: Error bars show s.e.m.. (E) Cell tracker (red)-stained primary microglia were seeded on top of a collagen matrix in 35 mm glass bottom dishes. One day after ctrl or 300 ng/ml WNT-5A stimulation, invasion was observed by confocal microscopy and Z-stacking using a Zeiss LSM710 microscope and subsequent analysis with the Bitplane Imaris software. The size of the collagen cube shown is 1,000 (Z) x 1,400 (Y) x 1,400 (X) μm. Three invasion experiments in the absence and presence of the MEK1/2 inhibitor SL327 were quantified. Data are presented in a bar graph (F). *, P < 0.05; error bars show s.e.m.. (G) cDNA of ctrl stimulated (−) and WNT-5A stimulated (+) primary microglia was analyzed by QPCR for expression of inflammatory genes. At least three independent experiments are summarized. Gene expression is normalized to the housekeeping gene GAPDH and expressed as arbitrary units (2-delta ct). *, P < 0.05; **, P < 0.01. COX-2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; n, number; s.e.m., standard error of the mean. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af-410-na_mouse-tnf-alpha-affinity-purified-polyclonal-ab-41202410331929.jpg)
![N/A TNF-alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_DY510_ELISA_2079.jpg "N/A TNF-alpha [Biotin]")
![Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0007.jpg "Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, P < 0.001 versus NS group; &P < 0.001 versus low-dose group. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/mi/2013/432623/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0008.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected diffused immunostaining of this protein in the tested tissue. Staining was primarily observed in the epithelial cells and some cells showed membrane positivity also.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0005.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected immunopositivity of this protein in the tested tissue. Staining was primarily observed in the epithelial cells while some staining was present in mucosa muscularis also.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] - Effects of femoral artery occlusion on TNF-alpha signal in sensory nerves. (A) Seventy-two hours of femoral artery occlusion increased the levels of TNF-alpha in the DRG tissues as compared with them in DRG of control limbs (n = 6 in each group); & PTX given into the hindlimb muscles attenuated amplification of TNF-alpha in the DRG tissues of limbs with femoral artery occlusion (n = 8). ∗P < 0.05, occlusion group vs. control & occlusion with prior PTX. (B) Immunofluorescence was used to examine double-labeling for TNF-alpha & peripherin/NF-200 (n = 3). Peripherin was used to label DRG neurons that project thin C-fibers. NF200 was used to identify A-fibers of DRG neurons. Representative photomicrographs show co-existence of TNF-alpha & peripherin staining in DRG neurons (top panel), whereas few TNF-alpha & NF-200 staining were observed in DRG neurons (bottom panel). Arrows indicate representative positive cells for both TNF-alpha & peripherin after they were merged. Scale bar = 50 μm. (C) Representative bands (left panel) & averaged data (right panel), demonstrating that femoral artery occlusion upregulated protein expression TNF-alpha receptor subtype TNFR1, but not TNFR2. A significant difference in TNFR1 was seen between control & occluded groups. ∗P < 0.05 vs. control. n = 6 in each group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30374312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19532_rabbit-polyclonal-tnf-alpha-antibody-31020241618927.jpg)
![Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] - Immunoflorescence staining results of TNF-alpha (96 h). A). Lamella of gill tissue (arrow). Control group. IF. 20 μm. B) Positive reaction in lamellar cells of gill tissue (arrows). Low dose toxic group. IF. 20 μm. C) Positivity in lamellar cells of gill tissue (arrow). High dose toxic group. IF. 20 μm. D) Liver tissue, vena centralis (arrow). Control group. IF. 20 μm. E) Positive reactions in hepatocytes (arrows). Low dose toxic group. IF. 20 μm. F) Positive reactions in hepatocytes (arrows). High dose toxic group. IF. 20 μm. G) Weak positive reactions in neurons (arrows). Control group. IF. 20 μm. H) Weak positivity in neurons (arrows). Low dose toxic group. IF. 20 μm. I) Positivity in neurons (arrows). High dose toxic group. IF. 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321977), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19532_rabbit-polyclonal-tnf-alpha-antibody-310202416171446.jpg)
![N/A TNF-alpha [HRP]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_PTA00_ELISA_1061.jpg "N/A TNF-alpha [HRP]")
![N/A TNF-alpha [HRP]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_PTA00_ELISA_1062.jpg)
![N/A TNF-alpha [HRP]](http://images.novusbio.com/fullsize2/TNF-alpha_PTA00_ELISA_343.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)