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![Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20053-img0016.jpg "Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0015.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0014.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP2-20053-img0010.jpg)
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242091440.jpg)
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242063390.jpg)
![Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of C6 cells, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-15608-img0006.jpg "Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of C6 cells, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP3-15608] - Immunofluorescence analysis of U2OS cells using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15608-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0003.jpg)
![Chromatin Immunoprecipitation: Rad21 Antibody [NBP3-15608] - Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody (NBP3-15608) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/Rad21-Antibody-Chromatin-Immunoprecipitation-NBP3-15608-img0008.jpg)
![Western Blot: Rad21 Antibody [NBP3-15608] - Western blot analysis of extracts of various cell lines, using Rad21 antibody (NBP3-15608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-15608-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP3-15608] - Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Rabbit pAb (NBP3-15608) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP3-15608-img0002.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP3-15608] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 antibody (NBP3-15608). Western blot was performed from the immunoprecipitate using Rad21 antibody (NBP3-15608) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP3-15608-img0007.jpg)
![Western Blot: Rad21 Antibody [NBP1-83260] - Analysis in control (vector only transfected HEK293T lysate) and RAD21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP1-83260-img0009.jpg "Western Blot: Rad21 Antibody [NBP1-83260] - Analysis in control (vector only transfected HEK293T lysate) and RAD21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP1-83260] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83260-img0008.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP1-83260-img0012.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP1-83260-img0007.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP1-83260-img0010.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP1-83260-img0011.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP1-83260-img0013.jpg)
![Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.](http://images.novusbio.com/fullsize/Rad21-Antibody-CM110-2C10-Western-Blot-NB100-386-img0003.jpg "Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.")
![Simple Western: Rad21 Antibody (CM110-2C10) [NB100-386] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad21-Antibody-CM110-2C10-Simple-Western-NB100-386-img0004.jpg)
![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-2120249393515.jpg "Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939177.jpg)
![Western Blot: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024939148.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody (HL2171) - Azide and BSA Free [NBP3-25619] - Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Rad21 stained by Rad21 antibody [HL2171] (NBP3-25619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25619_rabbit-rad21-mab-hl2171-azide-and-bsa-free-212024941456.jpg)
![Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Rad21-Antibody-NB100-203-img0010.jpg "Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Rad21 Antibody [NB100-203] - Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Rad21 antibody NB100-203 (Lot NB100-203-8) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot NB100-203-6). For blotting immunoprecipitated Rad21, rabbit anti-Rad21 recombinant monoclonal antibody NB100-203 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.Previous](http://images.novusbio.com/fullsize/Rad21-Antibody-NB100-203-img0011.jpg)
![Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiRad21 recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-14677-img0002.jpg "Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiRad21 recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO,GaMG, MCF-7, A-549, and U2OS cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-Rad21recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 seconds. Lower panel: Rabbit antiMED1 recombinant monoclonal antibody.](http://images.novusbio.com/fullsize/Rad21-Antibody-Western-Blot-NBP3-14677-img0001.jpg)
![Western Blot: Rad21 Antibody (9H0H6) [NBP3-15892] - Western blot analysis of extracts of various cell lines, using Rad21 Rabbit mAb (NBP3-15892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Rad21-Antibody-9H0H6-Western-Blot-NBP3-15892-img0001.jpg "Western Blot: Rad21 Antibody (9H0H6) [NBP3-15892] - Western blot analysis of extracts of various cell lines, using Rad21 Rabbit mAb (NBP3-15892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [NBP3-15892] - Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody (NBP3-15892) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/Rad21-Antibody-9H0H6-Chromatin-Immunoprecipitation-NBP3-15892-img0002.jpg)
![Western Blot: Rad21 Antibody (SR2172) [NBP3-21658] - Western blot analysis using NBP3-21658 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21658_rabbit-rad21-mab-sr2172-672023106285.jpg "Western Blot: Rad21 Antibody (SR2172) [NBP3-21658] - Western blot analysis using NBP3-21658 in HeLa cell lysate.")
![Western Blot: Rad21 Overexpression Lysate (Adult Normal) [NBL1-15114] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad21.](http://images.novusbio.com/fullsize/Rad21-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15114-img0002.jpg "Western Blot: Rad21 Overexpression Lysate (Adult Normal) [NBL1-15114] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad21.")