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ERK2 Antibody [Unconjugated]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

  • Size / Catalog#QtyPrice
    AF1230-SP
    25 ug
    $149.00
    5-7 Business Days
    AF1230
    100 ug
    $399.00
    1-3 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
Shipping $45.00 per order
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Support & Research

ERK2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ERK2
Met1-Ser360
Accession # P28482
Specificity
Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK1
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Knockout Validated
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1230 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK2 Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK
  • ERK2
  • ERK-2
  • ERK2MAP kinase isoform p42
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAPK 1
  • MAPK1
  • MAPK2
  • mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • p42-MAPK
  • PRKM1
  • PRKM1MAPK 2
  • PRKM2
  • protein tyrosine kinase ERK2

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAPK1
Uniprot