Melanoma HMB45 + MART1 Antibody (HMB45 + M2-7C10 + M2-9E3)

HMB45 - Pigmented melanoma metastases from LNMART-1 - Recombinant human MART-1 protein

Cytoplasmic

The HMB-45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells. It is also thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas.

IgG Kappa

Monoclonal

Mouse

MLANA

Tissue culture supernatant

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IHC:For an ABC system, incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, the staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol