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NBP1-91258

Fibronectin Antibody - BSA Free

(11 Reviews)

(52 Publications)

0.1 ml

NBP1-91258

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884/) licensed under a CC-BY license.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.

Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.

Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.

Simple Western: Fibronectin Antibody - BSA Free [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. The mice were sacrificed at 14 days after surgery to collect obstructed kidneys for histological analysis, Western blot and real-time RT-PCR analysis.(C) Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884), licensed under a CC-BY licence.

LC depressed both the protein and mRNA level of fibronectin via depletion of peritoneal M2. Values were expressed as the mean +/- SD. (D) Immunofluorescence staining of fibronectin in the four groups. Blue corresponds to nuclear staining, and red corresponds to fibronectin staining. #p < 0.05 vs. NC and LC group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23685870), licensed under a CC-BY licence.

Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. (A) Left; HDF were subjected to IR (10 Gy) and allowed to recover for 1 h. Association between A-type lamins and 53BP1 was assessed by immunoprecipitation of endogenous lamin A/C followed by immunoblotting with 53BP1 and lamin A/C antibodies. WCE, whole cell extract, IP: immunoprecipitates. WCE represents 5% input. Right; endogenous lamin A/C was immunoprecipitated and supernatants or pellets were analysed by immunoblotting for interaction with JMJD2A. (B) U2OS/GFP-lamin A cells were pretreated with caffeine (20 mm) for 1 h before exposure to IR (10 Gy, 1 h recovery). Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analysed by immunoblotting using 53BP1 and GFP antibodies. WCE represents 1% input. (C) As in (C) except cells were pretreated with 10 μm ATMi for 1 h before IR. WCE represents 1% input. (D) U2OS/GFP-lamin A cells were subjected to laser micro-irradiation, fixed 1 h later and immunostained with gamma -H2AX antibody. Scale bar, 10 μm. (E) U2OS cells were transfected with siCTRL or siLMNA and subjected to laser micro-irradiation, fixed 15 min later and then processed for immunofluorescence with gamma -H2AX and 53BP1 antibodies. Scale bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25645366), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-91258AF350
Alexa Fluor 405	NBP1-91258AF405
Alexa Fluor 488	NBP1-91258AF488
Alexa Fluor 532	NBP1-91258AF532
Alexa Fluor 594	NBP1-91258AF594
Alexa Fluor 647	NBP1-91258AF647
Alexa Fluor 700	NBP1-91258AF700
Alexa Fluor 750	NBP1-91258AF750
Allophycocyanin	NBP1-91258APC
Biotin	NBP1-91258B
DyLight 350	NBP1-91258UV
DyLight 405	NBP1-91258V
DyLight 488	NBP1-91258G
DyLight 550	NBP1-91258R
DyLight 594	NBP1-91258DL594
DyLight 650	NBP1-91258C
DyLight 680	NBP1-91258FR
DyLight 755	NBP1-91258IR
FITC	NBP1-91258F
HRP	NBP1-91258H
Janelia Fluor 549	NBP1-91258JF549
Janelia Fluor 646	NBP1-91258JF646
PE	NBP1-91258PE
PerCP	NBP1-91258PCP
mFluor Violet 450 SE	NBP1-91258MFV450
mFluor Violet 500 SE	NBP1-91258MFV500
mFluor Violet 610 SE	NBP1-91258MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-91258SS

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NB100-77417

CD45 Antibody (30-F11) - BSA Free

(5 Reviews)

(43 Publications)

0.5 mg

NB100-77417

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.

Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77417AF350
Alexa Fluor 405	NB100-77417AF405
Alexa Fluor 488	NB100-77417AF488
Alexa Fluor 532	NB100-77417AF532
Alexa Fluor 594	NB100-77417AF594
Alexa Fluor 647	NB100-77417AF647
Alexa Fluor 700	NB100-77417AF700
Alexa Fluor 750	NB100-77417AF750
Allophycocyanin	NB100-77417APC
Biotin	NB100-77417B
DyLight 350	NB100-77417UV
DyLight 405	NB100-77417V
DyLight 488	NB100-77417G
DyLight 550	NB100-77417R
DyLight 594	NB100-77417DL594
DyLight 650	NB100-77417C
DyLight 680	NB100-77417FR
DyLight 755	NB100-77417IR
FITC	NB100-77417F
HRP	NB100-77417H
Janelia Fluor 549	NB100-77417JF549
Janelia Fluor 646	NB100-77417JF646
PE	NB100-77417PE
PerCP	NB100-77417PCP
mFluor Violet 450 SE	NB100-77417MFV450
mFluor Violet 500 SE	NB100-77417MFV500
mFluor Violet 610 SE	NB100-77417MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80652-0.1mg
BSA Free	NB100-77417SS
Azide and BSA Free	NBP2-80652

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NBP1-48309

N-Cadherin Antibody (13A9)

(2 Reviews)

(41 Publications)

0.1 ml

NBP1-48309

Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.

Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.

Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.

Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Polarized RPE cell model characterization. (D) Representative images of several characteristic RPE markers: Na+ K+ ATPase, PEDF, E-cadherin, N-cadherin, claudin 19 and occludin (green); DAPI (blue); scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29269042), licensed under a CC-BY licence.

Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) and HIF-2 alpha (D–F) of tissue extracts from the cortex and outer and inner medulla of the left kidneys of rats 24 h and 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, and PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) and time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-48309AF350
Alexa Fluor 405	NBP1-48309AF405
Alexa Fluor 488	NBP1-48309AF488
Alexa Fluor 532	NBP1-48309AF532
Alexa Fluor 594	NBP1-48309AF594
Alexa Fluor 647	NBP1-48309AF647
Alexa Fluor 700	NBP1-48309AF700
Alexa Fluor 750	NBP1-48309AF750
Allophycocyanin/Cy7	NBP1-48309APCCY7
Biotin	NBP1-48309B
DyLight 350	NBP1-48309UV
DyLight 405	NBP1-48309V
DyLight 488	NBP1-48309G
DyLight 550	NBP1-48309R
DyLight 594	NBP1-48309DL594
DyLight 650	NBP1-48309C
DyLight 680	NBP1-48309FR
DyLight 755	NBP1-48309IR
FITC	NBP1-48309F
HRP	NBP1-48309H
Janelia Fluor 549	NBP1-48309JF549
Janelia Fluor 646	NBP1-48309JF646
PE/Atto594	NBP1-48309PEATT594
PE/Cy5.5	NBP1-48309PECY55
PE/Cy7	NBP1-48309PECY7
mFluor Violet 450 SE	NBP1-48309MFV450
mFluor Violet 500 SE	NBP1-48309MFV500
mFluor Violet 610 SE	NBP1-48309MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80868

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NB200-540

Complement C3 Antibody (11H9) - BSA Free

(2 Reviews)

(30 Publications)

0.1 ml

NB200-540

Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.

Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.

Reactivity:

Mu, Ec

Species Glossary

Reactivity: Mu, Ec

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-540AF350
Alexa Fluor 405	NB200-540AF405
Alexa Fluor 488	NB200-540AF488
Alexa Fluor 532	NB200-540AF532
Alexa Fluor 594	NB200-540AF594
Alexa Fluor 647	NB200-540AF647
Alexa Fluor 700	NB200-540AF700
Alexa Fluor 750	NB200-540AF750
Allophycocyanin	NB200-540APC
Biotin	NB200-540B
DyLight 350	NB200-540UV
DyLight 405	NB200-540V
DyLight 488	NB200-540G
DyLight 550	NB200-540R
DyLight 594	NB200-540DL594
DyLight 650	NB200-540C
DyLight 680	NB200-540FR
DyLight 755	NB200-540IR
FITC	NB200-540F
HRP	NB200-540H
Janelia Fluor 549	NB200-540JF549
Janelia Fluor 646	NB200-540JF646
PE	NB200-540PE
PerCP	NB200-540PCP
mFluor Violet 450 SE	NB200-540MFV450
mFluor Violet 500 SE	NB200-540MFV500
mFluor Violet 610 SE	NB200-540MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-540SS
Azide and BSA Free	NBP2-80677

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NB600-1071

CD34 Antibody (MEC 14.7)

(28 Publications)

0.1 ml

NB600-1071

Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as a negative control.

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Notably, the antibody specifically stained the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the colon.

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the heart tissue.

Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1071AF350
Alexa Fluor 405	NB600-1071AF405
Alexa Fluor 488	NB600-1071AF488
Alexa Fluor 532	NB600-1071AF532
Alexa Fluor 594	NB600-1071AF594
Alexa Fluor 647	NB600-1071AF647
Alexa Fluor 700	NB600-1071AF700
Alexa Fluor 750	NB600-1071AF750
Allophycocyanin	NB600-1071APC
Allophycocyanin/Cy7	NB600-1071APCCY7
Biotin	NB600-1071B
DyLight 350	NB600-1071UV
DyLight 405	NB600-1071V
DyLight 488	NB600-1071G
DyLight 550	NB600-1071R
DyLight 594	NB600-1071DL594
DyLight 650	NB600-1071C
DyLight 680	NB600-1071FR
DyLight 755	NB600-1071IR
FITC	NB600-1071F
HRP	NB600-1071H
Janelia Fluor 549	NB600-1071JF549
Janelia Fluor 646	NB600-1071JF646
PE	NB600-1071PE
PE/Atto594	NB600-1071PEATT594
PE/Cy5.5	NB600-1071PECY55
PE/Cy7	NB600-1071PECY7
PerCP	NB600-1071PCP
mFluor Violet 450 SE	NB600-1071MFV450
mFluor Violet 500 SE	NB600-1071MFV500
mFluor Violet 610 SE	NB600-1071MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80642

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NB100-74350

Aggrecan Neoepitope Antibody - BSA Free

(29 Publications)

0.1 ml

NB100-74350

Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.

Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in U2OS cell lysate.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-74350AF350
Alexa Fluor 405	NB100-74350AF405
Alexa Fluor 488	NB100-74350AF488
Alexa Fluor 532	NB100-74350AF532
Alexa Fluor 594	NB100-74350AF594
Alexa Fluor 647	NB100-74350AF647
Alexa Fluor 700	NB100-74350AF700
Alexa Fluor 750	NB100-74350AF750
Allophycocyanin	NB100-74350APC
Allophycocyanin/Cy7	NB100-74350APCCY7
Biotin	NB100-74350B
DyLight 350	NB100-74350UV
DyLight 405	NB100-74350V
DyLight 488	NB100-74350G
DyLight 550	NB100-74350R
DyLight 594	NB100-74350DL594
DyLight 650	NB100-74350C
DyLight 680	NB100-74350FR
DyLight 755	NB100-74350IR
FITC	NB100-74350F
HRP	NB100-74350H
Janelia Fluor 549	NB100-74350JF549
Janelia Fluor 646	NB100-74350JF646
PE	NB100-74350PE
PE/Atto594	NB100-74350PEATT594
PE/Cy5.5	NB100-74350PECY55
PE/Cy7	NB100-74350PECY7
PerCP	NB100-74350PCP
mFluor Violet 450 SE	NB100-74350MFV450
mFluor Violet 500 SE	NB100-74350MFV500
mFluor Violet 610 SE	NB100-74350MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-74350SS

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NBP1-47491

VCAM-1/CD106 Antibody (6G9) - BSA Free

(2 Reviews)

(5 Publications)

0.1 ml

NBP1-47491

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.

Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Photomicrographs of two separate gut sections from a patient with EHEC colitis.Panels (A) and (B) are stained with CD31 to enumerate endothelium lining the vessels (40× magnification). (C) and (D) are stained to show VCAM-1 expression in endothelium, indicating inflammatory activation (40× magnification).

Small interference RNA targeting Raptor and Rictor disrupts BMAL1 accumulation in HNSCCTargeted disruption of Raptor (A-B) and Rictor (C-D) using siRNA results in a dose-dependent downregulation of BMAL1 in HNSCC cells. E. Disruption of PTEN by protein oxidation causes activation of mTOR signaling, resulting in accumulation of BMAL1. Notably, inhibition of mTOR signaling, particularly mTORC1 and mTORC2, results in restoration of normal BMAL1 levels in the epidermis of mice and head and neck cancer cells. These results demonstrate a novel role for mTOR in regulating nuclear levels of the core clock gene BMAL1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Identification of an HDR-enhancing fragment of 53BP1. a Schematic diagram of full-length human 53BP1 protein showing the minimal focus forming region including the oligomerization domain (OD), a glycine-arginine rich (GAR) motif, a tandem Tudor domain, and the ubiquitin-dependent recruitment (UDR) motif. BRCT repeats at the extreme C-terminus are present. b Different truncated 53BP1 proteins, DN1, DN1S, DN2, DN3, and DN4, were tested for their relative expression by western blot using anti-HA antibody in HeLa cells transduced with empty vector (mock) or lentiviral vectors encoding HA-tagged 53BP1 fragments DN1, DN1S, DN2, DN3, or DN4. Actin is shown as a loading control. c, e, g Representative immunofluorescence (IF) images of HA-tagged DN1 and DN1S at nuclear foci. Cells were either exposed to 2 Gy IR or no IR (control) and fixed 2 h later. Scale bars represent 20 μm. c Representative IF images showing co-localization of HA-tagged DN1 or DN1S with endogenous 53BP1 foci. d Number (no.) of HA+ (DN1S) foci, co-localized HA+/endogenous 53BP1+ (co-localization) foci, or endogenous 53BP1+ (E-53BP1) only foci per IR-treated HeLa cell expressing DN1S. Individual quantifications of foci from 50 cells in each group are shown. Red lines are drawn at the mean number of foci per positive cell. e Representative IF images showing HA-tagged DN1 or DN1S and RIF1 recruitment to IR-induced DNA repair foci. f Quantification of cells with ≥3 RIF1 foci in control cells or IR cells with DN1, DN1S or without vector (mock). g Representative IF images showing HA-tagged DN1S and BRCA1 recruitment to DNA repair foci in control cells. h Quantification of the number of cells with ≥3 BRCA1 foci in control cells or IR cells, with DN1, DN1S or without vector (mock). In panels f and g, data are presented as the mean ± SEM of counts of 150 cells each, from three independent fields, and black circles indicate individual counts. Statistics for panels d, f, and g: ANOVA. ns indicates not significant, *p < 0.05, ***p < 0.001, and ****p < 0.0001. Source data is available in Source Data file Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31253785), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-47491AF350
Alexa Fluor 405	NBP1-47491AF405
Alexa Fluor 488	NBP1-47491AF488
Alexa Fluor 532	NBP1-47491AF532
Alexa Fluor 594	NBP1-47491AF594
Alexa Fluor 647	NBP1-47491AF647
Alexa Fluor 700	NBP1-47491AF700
Alexa Fluor 750	NBP1-47491AF750
Biotin	NBP1-47491B
DyLight 350	NBP1-47491UV
DyLight 405	NBP1-47491V
DyLight 488	NBP1-47491G
DyLight 550	NBP1-47491R
DyLight 594	NBP1-47491DL594
DyLight 650	NBP1-47491C
DyLight 680	NBP1-47491FR
DyLight 755	NBP1-47491IR
FITC	NBP1-47491F
HRP	NBP1-47491H
Janelia Fluor 549	NBP1-47491JF549
Janelia Fluor 646	NBP1-47491JF646
mFluor Violet 450 SE	NBP1-47491MFV450
mFluor Violet 500 SE	NBP1-47491MFV500
mFluor Violet 610 SE	NBP1-47491MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-47491SS
Azide and BSA Free	NBP2-81005

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NBP1-47386

CD44 Antibody (8E2F3) - BSA Free

(1 Review)

(19 Publications)

0.1 ml

NBP1-47386

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of pSTAT3, STAT3, and CD44 (NBP1-47386) in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047/) licensed under a CC-BY license.

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).

Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

$Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. d) Western blot analysis of pSTAT3, STAT3, and CD44 in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047), licensed under a CC-BY licence.

CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP1-47386B) at 2 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rb(-), Pm, Ca, Eq

Species Glossary

Reactivity: Hu, Mu, Rb(-), Pm, Ca, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-47386AF350
Alexa Fluor 405	NBP1-47386AF405
Alexa Fluor 488	NBP1-47386AF488
Alexa Fluor 532	NBP1-47386AF532
Alexa Fluor 594	NBP1-47386AF594
Alexa Fluor 647	NBP1-47386AF647
Alexa Fluor 700	NBP1-47386AF700
Alexa Fluor 750	NBP1-47386AF750
Allophycocyanin	NBP1-47386APC
Allophycocyanin/Cy7	NBP1-47386APCCY7
Biotin	NBP1-47386B
DyLight 350	NBP1-47386UV
DyLight 405	NBP1-47386V
DyLight 488	NBP1-47386G
DyLight 550	NBP1-47386R
DyLight 594	NBP1-47386DL594
DyLight 650	NBP1-47386C
DyLight 680	NBP1-47386FR
DyLight 755	NBP1-47386IR
FITC	NBP1-47386F
HRP	NBP1-47386H
Janelia Fluor 549	NBP1-47386JF549
Janelia Fluor 646	NBP1-47386JF646
PE	NBP1-47386PE
PE/Atto594	NBP1-47386PEATT594
PE/Cy5.5	NBP1-47386PECY55
PE/Cy7	NBP1-47386PECY7
PerCP	NBP1-47386PCP
mFluor Violet 450 SE	NBP1-47386MFV450
mFluor Violet 500 SE	NBP1-47386MFV500
mFluor Violet 610 SE	NBP1-47386MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-47386SS
Azide and BSA Free	NBP2-80650

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NB110-58359

TAZ/WWTR1 Antibody - BSA Free

(1 Review)

(18 Publications)

0.1 ml

NB110-58359

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.

Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean ± SD analyzed by one-way analysis of variance. ∗∗P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32328124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The expression of BDNF-TrkB signaling in the SDH of CYP-induced cystitis. a Changes of the mechanical threshold in CYP-induced cystitis model. Compared to that in the control group, the mechanical threshold of the cystitis group decreased significantly after the CYP injection and remained low until day 17, and the minimum threshold value was reached on day 12. The expression of b BDNF, c TrkB, and dp-TrkB were evaluated by western blots. Compared to the control group, they were upregulated on days 8, 12, and 17. e Immunofluorescence double staining assay of BDNF and p-TrkB in the SDH. BDNF and p-TrkB (red), NeuN, GFAP, and OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. And TrkB receptors expressed in neurons, microglia, and astrocytes. The white dotted lines in picture “BDNF/NeuN” showed the laminate of the SDH according to Rexed and Steiner. Scale bar = 100 μm. All data were calculated as mean ± SEM (n = 5 per group). *p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Periodontitis-derived periodontal ligament cells and tissues were at high levels of oxidative stress and apoptosis. (a) The related gene expressions of oxidative stress, apoptosis, and some of the TRP families in healthy and periodontitis-derived periodontal ligament cells (PDLCs and P-PDLCs) (n = 3). (b, c) Western blot and semiquantitative statistical analysis of oxidative stress, apoptosis, and TRPA1 in PDLCs and P-PDLCs. (n = 3). d, Flow cytometry analysis of PDLCs and P-PDLCs (n = 4). (e, f) H&E staining (white star represent immune cell infiltration), immunohistochemistry and immunofluorescence staining, and semiquantitative statistical analysis of periodontitis and healthy derived periodontal ligament tissues (PDLTs and P-PDLTs) (n = 3). Data analysis was performed by using Student's t-test (∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001). The data are presented as the mean ± SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Subcellular localization of BEST1 and surface Ca2+-dependent Cl- current in patient-derived iPSC-RPEs.(A) Western blots show similar BEST1 expression levels in WT and patient-derived iPSC-RPEs. Each sample was from one cell lysis (BEST1 and beta -actin, RPE65 and CRALBP were on two gels, respectively). (B) Confocal images showing diminished plasma membrane localizations of BEST1 P274R, and normal plasma membrane localization of BEST1 I201T. Scale bar,15 μm. (C) Representative current traces recorded from patient iPSC-RPEs at 1.2 μM [Ca2+]i. Scale bar, 500 pA, 150 ms. (D) Population steady-state current-voltage relationships in BEST1 WT (●), P274R (▲) and I201T (▼) iPSC-RPEs at 1.2 μM [Ca2+]i; n = 5–6 for each point. ∗p<0.05 (2 × 10−7 for P274R and 6 × 10−4 for I201T) compared to WT using two-tailed unpaired Student t test. Insert, confocal images showing P274R iPSC-RPE in bright field. Scale bar,10 μm. (E) CaCC currents in BEST1 P274R patient iPSC-RPE were rescued by complementation with WT BEST1-GFP. Complementation (▲, n = 5–6 for each point), compared to BEST1 P274R (▲, n = 3–5 for each point), and WT (●). The plots were fitted to the Hill equation. Insert, confocal images showing P274R iPSC-RPE complemented with WT BEST1-GFP expressed from a BacMam baculoviral vector. Scale bar,10 μm. (F) Ca2+-dependent currents in BEST1 I201T iPSC-RPE (▼) compared to WT iPSC-RPE (●). Steady-state current density recorded at +100 mV plotted vs. free [Ca2+]i; n = 5–6 for each point. The plots were fitted to the Hill equation. See also Figure 4—figure supplement 1 and Figure 1—source data 1.CaCC currents in BEST1 patient iPSC-RPEs.(A–C) P274R patient iPSC-RPE were rescued by complementation with WT BEST1-GFP at 1.2 μM [Ca2+]i. (A) Representative current traces recorded from P274R patient iPSC-RPE over-expressing WT BEST1-GFP. Scale bar, 1 nA, 100 ms. (B) Population steady-state current-voltage relationships in BEST1-GFP complementation (▲), compared to BEST1 P274R (▲) and WT (●); n = 5–6 for each point. #p<0.05 compared to WT (2 × 10−7) or complementation (0.01) using one-way ANOVA and Bonferroni post hoc analyses. (C) Bar chart showing the steady-state current amplitudes at 1.2 μM [Ca2+]i and 1.2 μM [Ca2+]i +100μM NFA in P274R patient iPSC-RPE over-expressing BEST1-GFP; n = 5–6. ∗p<0.05 compared to current amplitudes at 1.2 μM [Ca2+]i, using two-tailed unpaired Student t test. (D) Normalized Ca2+-dependent currents in BEST1 I201T iPSC-RPE (▼) compared to WT iPSC-RPE (●). The plots were fitted to the Hill equation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29063836), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-58359AF350
Alexa Fluor 405	NB110-58359AF405
Alexa Fluor 488	NB110-58359AF488
Alexa Fluor 532	NB110-58359AF532
Alexa Fluor 594	NB110-58359AF594
Alexa Fluor 647	NB110-58359AF647
Alexa Fluor 700	NB110-58359AF700
Alexa Fluor 750	NB110-58359AF750
Allophycocyanin	NB110-58359APC
Biotin	NB110-58359B-100ul
DyLight 350	NB110-58359UV
DyLight 405	NB110-58359V-100ul
DyLight 488	NB110-58359G-100ul
DyLight 550	NB110-58359R-100ul
DyLight 594	NB110-58359DL594
DyLight 650	NB110-58359C-100ul
DyLight 680	NB110-58359FR
DyLight 755	NB110-58359IR
FITC	NB110-58359F-100ul
HRP	NB110-58359H-100ul
Janelia Fluor 549	NB110-58359JF549
Janelia Fluor 646	NB110-58359JF646
PE	NB110-58359PE
PerCP	NB110-58359PCP
mFluor Violet 450 SE	NB110-58359MFV450
mFluor Violet 500 SE	NB110-58359MFV500
mFluor Violet 610 SE	NB110-58359MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-58359SS

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NB100-77668

Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

(25 Publications)

0.5 mg

NB100-77668

Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Mouse embryonic cell lysate (A). Antibody at 2 ug/mL.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77668AF350
Alexa Fluor 405	NB100-77668AF405
Alexa Fluor 488	NB100-77668AF488SS
Alexa Fluor 488	NB100-77668AF488
Alexa Fluor 532	NB100-77668AF532
Alexa Fluor 594	NB100-77668AF594
Alexa Fluor 647	NB100-77668AF647
Alexa Fluor 700	NB100-77668AF700
Alexa Fluor 750	NB100-77668AF750
Allophycocyanin	NB100-77668APC-500ul
Allophycocyanin	NB100-77668APC-0.1ml
Allophycocyanin/Cy7	NB100-77668APCCY7
Biotin	NB100-77668B-100ul
DyLight 350	NB100-77668UV-0.1ml
DyLight 350	NB100-77668UV
DyLight 405	NB100-77668V
DyLight 405	NB100-77668V-0.1ml
DyLight 488	NB100-77668G-0.1ml
DyLight 488	NB100-77668G
DyLight 550	NB100-77668R
DyLight 594	NB100-77668DL594
DyLight 650	NB100-77668C
DyLight 680	NB100-77668FR
DyLight 680	NB100-77668FR-0.1ml
DyLight 755	NB100-77668IR-0.1ml
DyLight 755	NB100-77668IR
FITC	NB100-77668F-500ul
HRP	NB100-77668H
Janelia Fluor 549	NB100-77668JF549
Janelia Fluor 646	NB100-77668JF646
PE	NB100-77668PE
PE	NB100-77668PE-0.1ml
PE/Atto594	NB100-77668PEATT594
PE/Cy5.5	NB100-77668PECY55
PE/Cy7	NB100-77668PECY7
PerCP	NB100-77668PCP
PerCP	NB100-77668PCP-0.1ml
mFluor Violet 450 SE	NB100-77668MFV450
mFluor Violet 500 SE	NB100-77668MFV500
mFluor Violet 610 SE	NB100-77668MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-77668SS
Azide and BSA Free	NBP2-80903

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NB600-504

Aggrecan Antibody (BC-3) - BSA Free

(21 Publications)

0.1 mg

NB600-504

Western Blot: Aggrecan Antibody (BC-3) [NB600-504] - Expression levels of related genes during differentiation induction in the RCCS environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during differentiation induction. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.

Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. and B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), and exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) and HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, and actin. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Correlation between HIF-alpha , FASN Protein Levels and HIF Target Gene Expression in Adipogenesis and Prostate Cancer. (A) Downregulation of HIF-alpha protein levels by 25-OH treatment. HCT116, HeLa and 293 T cells were treated with 25-OH for 72 h and incubated for the last 4 h in 1% oxygen. HIF-alpha levels were analyzed by western blotting. (B) Hif-alpha activation during differentiation of 3T3-L1 cells into adipocytes. Confluent 3T3-L1 cells were cultured for 14 days in regular medium (1), or differentiation medium in the presence of insulin alone (2) or with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (3). Fasn, Hif-alpha and Redd1 expression was analyzed by western blotting. Lipid accumulation was monitored by staining with Oil-Red O. (C) Time-dependent changes of Hif target gene expression during 3T3-L1 differentiation. Inducers of differentiation were added at Day 2. mRNAs of the Hif target genes were measured by qPCR in biological triplicates and technical duplicates as a total of 6 samples and normalized against the reference gene Tbp and Day 2 samples. Data plots with p-value larger than 0.01 were indicated by red brackets and a red arrow additionally showing Vegf alpha at Day 2. Lipid staining results with Oil-Red O are shown under the graph for each time point. (D) Stability of Hif-1 alpha protein. Stability was measured after oxygen concentration shift (1.0% to 21% oxygen) for undifferentiated cells, cycloheximide (CHX) addition for undifferentiated cells in hypoxia, or cycloheximide addition for differentiated cells in normoxia. (E) Gene expression profiles of prostate cancer patients obtained from IST Online (MediSapiens) was analyzed, and mRNA levels of several HIF target genes were examined in correlation with FASN levels. Patient samples co-overexpressed with FASN are indicated with a horizontal bar. M stage: metastasis, PSA: prostate-specific antigen. Experiments were repeated twice (A,B,D), and the results were reproduced. The qPCR experiment (C) was performed once with biological triplicates. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Sirtuin-3 (SIRT3) is overexpressed in oral squamous cell carcinoma (OSCC). (A) Immunoblots reveal the levels of sirtuins (SIRT1-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). beta -Actin served as loading control. (B) These representative samples show (Top) SIRT3 expression levels in OSCC (tongue) and (Bottom) in normal tissues. Immunoglobulin G (IgG) served as a negative control. Scale bars = 200 μm for low-magnification photomicrographs (left column); 50 μm for high-magnification photomicrographs (middle and right columns). (C) These charts illustrate the percentage of normal and OSCC tissue specimens that expressed SIRT3 (Top) in all samples and (Bottom) in tongue samples, as determined by immunohistochemical staining. Note that SIRT3 staining intensities were designated as low (L) or high (H) from the data provided in Table 1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21472714), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry of seven in absentia homolog 2 (SIAH2) gene in normal, in situ and invasive breast carcinomas. (A) Occasional nuclear positivity (arrows) in luminal cells in the terminal duct lobular unit. (B) Moderate to strong staining of SIAH2 in the nucleus of a small proportion of the cell in a high nuclear grade ductal carcinoma in situ with comedo necrosis. (C) Occasional weak to moderate SIAH2 (arrows) staining in a luminal type ductal carcinoma. (D) Strong SIAH2 staining in all nuclei in this basal-like breast carcinoma. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21306611), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Rb, Sh

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-504AF350
Alexa Fluor 532	NB600-504AF532
Alexa Fluor 594	NB600-504AF594
Alexa Fluor 647	NB600-504AF647-0.1ml
Alexa Fluor 750	NB600-504AF750
Allophycocyanin	NB600-504APC
Biotin	NB600-504B-100ul
DyLight 350	NB600-504UV
DyLight 405	NB600-504V
DyLight 488	NB600-504G-100ul
DyLight 550	NB600-504R-100ul
DyLight 594	NB600-504DL594
DyLight 650	NB600-504C-100ul
DyLight 680	NB600-504FR
DyLight 755	NB600-504IR
FITC	NB600-504F
HRP	NB600-504H-100ul
Janelia Fluor 549	NB600-504JF549
Janelia Fluor 646	NB600-504JF646
PE	NB600-504PE
PerCP	NB600-504PCP
mFluor Violet 450 SE	NB600-504MFV450
mFluor Violet 500 SE	NB600-504MFV500
mFluor Violet 610 SE	NB600-504MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-504-0.025mg

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NBP2-22122

Endoglin/CD105 Antibody (3A9) - BSA Free

(3 Reviews)

(6 Publications)

0.1 ml

NBP2-22122

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.

Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.

ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22122AF350
Alexa Fluor 405	NBP2-22122AF405
Alexa Fluor 488	NBP2-22122AF488
Alexa Fluor 532	NBP2-22122AF532
Alexa Fluor 594	NBP2-22122AF594
Alexa Fluor 647	NBP2-22122AF647
Alexa Fluor 700	NBP2-22122AF700
Alexa Fluor 750	NBP2-22122AF750
Biotin	NBP2-22122B
DyLight 350	NBP2-22122UV
DyLight 405	NBP2-22122V
DyLight 488	NBP2-22122G
DyLight 550	NBP2-22122R
DyLight 594	NBP2-22122DL594
DyLight 650	NBP2-22122C
DyLight 680	NBP2-22122FR
DyLight 755	NBP2-22122IR
FITC	NBP2-22122F
HRP	NBP2-22122H
Janelia Fluor 549	NBP2-22122JF549
Janelia Fluor 646	NBP2-22122JF646
mFluor Violet 450 SE	NBP2-22122MFV450
mFluor Violet 500 SE	NBP2-22122MFV500
mFluor Violet 610 SE	NBP2-22122MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22122SS
Azide and BSA Free	NBP2-80719

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Fibronectin Antibody - BSA Free

CD45 Antibody (30-F11) - BSA Free

N-Cadherin Antibody (13A9)

Complement C3 Antibody (11H9) - BSA Free

CD34 Antibody (MEC 14.7)

Aggrecan Neoepitope Antibody - BSA Free

VCAM-1/CD106 Antibody (6G9) - BSA Free

CD44 Antibody (8E2F3) - BSA Free

TAZ/WWTR1 Antibody - BSA Free

Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Aggrecan Antibody (BC-3) - BSA Free

Endoglin/CD105 Antibody (3A9) - BSA Free