Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Western-Blot-NB100-97821-img0011.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - MCF-7 cells stained with Lysine (K)-specific Demethylase 5B antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NB100-97821-img0007.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody used at 1:500 (2ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NB100-97821-img0012.jpg)
![Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NB100-97821] - Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody NB100-97821 (lot NB100-97821-3) used for IP at 6 ug per reaction. JARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-97821-2). For blotting immunoprecipitated Jarid1B, NB100-97821 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunoprecipitation-NB100-97821-img0013.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining in human testis and skeletal muscle tissues . Corresponding KDM5B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining in human testis and skeletal muscle tissues . Corresponding KDM5B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0010.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0011.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0012.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human rectum shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0013.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunohistochemistry-Paraffin-NBP1-84352-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: JARID1B Antibody [NBP1-97310] - Jarid1B / KDM5B antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/JARID1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97310-img0003.jpg "Immunocytochemistry/Immunofluorescence: JARID1B Antibody [NBP1-97310] - Jarid1B / KDM5B antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: JARID1B Antibody [NBP1-97310] - IHC analysis of JARID1B in human breast cancer xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/JARID1B-Antibody-Immunohistochemistry-NBP1-97310-img0004.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - KDM5B monoclonal antibody (M02), clone 1G10. Analysis of KDM5B expression in HeLa.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-1G10-Western-Blot-H00010765-M02-img0010.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - KDM5B monoclonal antibody (M02), clone 1G10. Analysis of KDM5B expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-1G10-Immunocytochemistry-Immunofluorescence-H00010765-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-1G10-Immunohistochemistry-Paraffin-H00010765-M02-img0008.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of JARID1B expression in Hela NE.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-1G10-Western-Blot-H00010765-M02-img0001.jpg)
![ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-1G10-ELISA-H00010765-M02-img0011.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Western-Blot-NBP2-52969-img0008.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of MCF7 cells using the Anti-KDM5B monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunocytochemistry-Immunofluorescence-NBP2-52969-img0009.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human uterus shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0007.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human breast cancer shows strong nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0001.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human colorectal cancer shows moderate immunoreactivity in cancer cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0002.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human liver shows absence of immunoreactivity in hepatocytes (negative control).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0003.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0004.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate shows moderate immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0005.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0006.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Analysis using KDM5B mAb against human KDM5B (AA: 231-319) recombinant protein. (Expected MW is 36.2 kDa).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-7H3D7-Western-Blot-NBP2-52500-img0002.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Analysis using KDM5B mAb against human KDM5B (AA: 231-319) recombinant protein. (Expected MW is 36.2 kDa).")
![Flow Cytometry: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Analysis of MCF-7 cells using KDM5B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-7H3D7-Flow-Cytometry-NBP2-52500-img0001.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Against HEK293 (1) and KDM5B (AA: 231-319)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-7H3D7-Western-Blot-NBP2-52500-img0003.jpg)
![ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (7H3D7) [NBP2-52500] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-7H3D7-ELISA-NBP2-52500-img0004.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Western-Blot-NBP3-18678-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.")
![ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-ELISA-NBP3-18678-img0002.jpg)
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1147) [NBP2-34473] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1147-Western-Blot-NBP2-34473-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1147) [NBP2-34473] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP2-58615] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58615-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP2-58615] - Staining of human cell line U-2 OS shows localization to nucleus.")