![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-Western-Blot-NBP3-18678-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B (Jumonji, AT rich interactive domain 1B), using a KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KDM5B |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP3-18678)Find related products by research area.
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![ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-ELISA-NBP3-18678-img0002.jpg "ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-18678] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")