![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Western-Blot-NBP2-52969-img0008.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL1129 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE |
| Epitope | DGINSLERKLKRRLE |
| Predicted Species | Rat (91%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KDM5B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP2-52969)Find related products by research area.
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| Gene Symbol | KDM5B |
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of MCF7 cells using the Anti-KDM5B monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunocytochemistry-Immunofluorescence-NBP2-52969-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of MCF7 cells using the Anti-KDM5B monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human uterus shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0007.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human uterus shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human breast cancer shows strong nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0001.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human breast cancer shows strong nuclear immunoreactivity in tumor cells.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human colorectal cancer shows moderate immunoreactivity in cancer cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0002.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human colorectal cancer shows moderate immunoreactivity in cancer cells.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human liver shows absence of immunoreactivity in hepatocytes (negative control).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0003.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human liver shows absence of immunoreactivity in hepatocytes (negative control).")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0004.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate cancer shows strong nuclear positivity in tumor cells.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate shows moderate immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0005.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate shows moderate immunoreactivity in glandular cells.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-5B-KDM5B-JARID1B-Antibody-CL1129-Immunohistochemistry-Paraffin-NBP2-52969-img0006.jpg "Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")