Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) Summary
Immunogen |
KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGN |
Specificity |
JARID1B (1G10) |
Isotype |
IgG2a Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
KDM5B |
Purity |
Ascites |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:100 - 1:2000
- Immunocytochemistry/ Immunofluorescence
- Immunodiffusion
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:100 - 1:2000
- Western Blot 1:100 - 1:2000
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Publications |
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Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
In 1x PBS, pH 7.4 |
Preservative |
No Preservative |
Purity |
Ascites |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10)
Background
JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (H00010765-M02)(5)
Showing Publications 1 -
5 of 5.
Publications using H00010765-M02 |
Applications |
Species |
Veronika M, Simone B, Daisy H et al. The KDM5B and KDM1A lysine demethylases cooperate in regulating androgen receptor expression and signalling in prostate cancer. Front Cell Dev Biol. 2023-04-19 [PMID: 37152294] |
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Shigekawa Y, Hayami S, Ueno M et al. Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma. Oncotarget 2018-09-28 [PMID: 30344945] |
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Mitra D, Das PM, Huynh FC, Jones FE. Jumonji/ARID1 B (JARID1B) Protein Promotes Breast Tumor Cell Cycle Progression through Epigenetic Repression of MicroRNA let-7e. J Biol Chem. 2011-10-03 [PMID: 21969366] |
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Hayami S, Yoshimatsu M, Veerakumarasivam A et al. Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway. Mol Cancer. 2010-05-13 [PMID: 20226085] |
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Xiang Y, Zhu Z, Han G et al. JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer. Proc Natl Acad Sci U S A 2007-11-28 [PMID: 18048344] |
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Product General Protocols
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FAQs for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (H00010765-M02) (0)
Secondary Antibodies
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Isotype Controls
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