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Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10)

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Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - KDM5B monoclonal antibody (M02), clone 1G10. Analysis of KDM5B expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. Antibody ...read more
Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of JARID1B expression in Hela NE.
ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC
Clone
1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) Summary

Immunogen
KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGN
Specificity
JARID1B (1G10)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KDM5B
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunodiffusion
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
Publications
Read Publications using H00010765-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10)

  • CT31
  • CT31FLJ10538
  • EC 1.14.11
  • EC 1.14.11.-
  • homolog 1A
  • Jarid1B
  • JARID1BFLJ16281
  • Jumonji, AT rich interactive domain 1B (RBP2-like)
  • jumonji, AT rich interactive domain 1B
  • KDM5B
  • Lysine (K)specific Demethylase 5B
  • Lysine (K)-specific Demethylase 5B
  • PLU-1
  • PLU-1FLJ12459
  • PLU1FLJ23670
  • PUT1
  • RBBP2H1
  • RBBP2H1AFLJ12491
  • RBP2-H1
  • Retinoblastoma-binding protein 2 homolog 1

Background

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (H00010765-M02)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM5B