| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human KDM5B Leu819-Arg918 Accession # Q9UGL1 |
| Specificity | Detects human KDM5B in Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | KDM5B |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
KDM5B (also JARID1B, RBP2-H1 and PLU-1) is a 170-200 kDa member of the JARID1 histone demethylase family of proteins. It is a transcriptional repressor that is highly expressed in Sertoli cells and mammary epithelium during pregnancy, and interacts with BF-1 and PAX9. KDM5B is reported to demethylate tri- and di-methylated Lys4 on histone H3. It has no activity on Lys9 or 27. Human KDM5B is 1544 amino acids (aa) in length. It contains a jumonji N domain (aa 32-73), an ARID DNA binding region (aa 97-187), a PHD domain (aa 309-359), a jumonji C domain (aa 486-602), one C5HC2 motif (aa 692-745), and two consecutive PHD domains (aa 1176-1538). There are three utilized phosphorylation sites at Ser1383, 1384 and 1405.There are three potential splice variants. Two show a premature truncation after Pro1433 coupled to an alternative start site at either Met396 or Met159. A third contains a 36 aa insertion after Glu237. Over aa 819-918, human KDM5B shares 96% aa identity with mouse KDM5B.
Secondary Antibodies |
Isotype Controls |
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