| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75281 | |||
| DyLight 350 | NBP1-75635 | |||
| DyLight 488 | NBP1-75285 | |||
| DyLight 550 | NBP1-75636 | |||
| DyLight 594 | NBP1-75637 | |||
| DyLight 633 | NBP1-75638 | |||
| FITC | NBP1-75282 | |||
| HRP | NBP1-75283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75281 | |||
| Pre-adsorbed | NBP1-75282 | |||
| Pre-adsorbed | NBP1-75283 | |||
| Pre-adsorbed | NBP1-75285 | |||
| Pre-adsorbed | NBP1-75635 | |||
| Pre-adsorbed | NBP1-75636 | |||
| Pre-adsorbed | NBP1-75637 | |||
| Pre-adsorbed | NBP1-75638 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7570 | |||
| Pre-adsorbed | NB7569 | |||
| Pre-adsorbed | NB7568 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75399 | |||
| Pre-adsorbed | NBP1-75401 | |||
| Pre-adsorbed | NBP1-75402 | |||
| Pre-adsorbed | NBP1-75398-0.5mg | |||
| Pre-adsorbed | NBP1-75398JF549 | |||
| Pre-adsorbed | NBP1-75398JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7488 | |||
| Pre-adsorbed | NB7489 | |||
| Pre-adsorbed | NB7487B | |||
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0021.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Also shown in this 2-color STED image is a-tubulin monoclonal antibody [MOUSE] detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody (colored RED). Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4C. Secondary antibody: DyLight 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0018.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72732-img0026.jpg)
![SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - Non-Reduced.Lane 2: Goat anti-Rabbit IgG - Reduced.Load: 1.0 ug per lane.Predicted/Observed size: 25 & 55 kDa - Reduced, 160 kDa - Non-Reduced for Goat IgG.Other Band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-SDS-Page-NBP1-72732-img0025.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72739-img0005.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy and high resolution Stimulated Emission Depletion (STED) Microscopy. Both Dylight and Atto conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit C. (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0002.jpg)
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Staining of paraffin embedded mouse tissue. Goat anti-Mouse DyLight 488 was used as a secondary antibody. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Paraffin-NBP1-72739-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy (upper images) and high resolution Stimulated Emission Depletion (STED) Microscopy (lower images). Both Dylight and ATTO conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0001.jpg)
![Immunohistochemistry-Frozen: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - MDA-MB-231 cells were stained. Goat anti-Mouse DyLight 488 was used as a secondary antibody and DRAQ5 was used for nuclear stain. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-72739-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb, Rt, Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated.Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at 4C.Secondary antibody: N/A.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT. Image using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0006.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - A three-fold serial dilution of Mouse IgG starting at 200ug was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto 1 Hour at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0009.jpg)
![ELISA: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - ELISA results of purified Goat anti-Mouse IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Gt-anti-Donkey HRP conjugate 616-1302, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72739-img0011.jpg)
![Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72763-img0009.jpg "Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.")
![ELISA: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - ELISA Results of Purified Guinea Porcine Anti-Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 ug of Rabbit IgG [Red Line], Human IgG [Green Line], Goat IgG [Blue Line], Mouse IgG [Purple Line]. The working dilution is 1:17,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Guinea Porcine mx10 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72763-img0010.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0006.jpg "Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.")
![Immunohistochemistry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of mouse tissue. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-NBP1-75279-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human pancreatic cancer cells. Note: Need to be careful about bleeding into different filters since it might interfere with FITC. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75279-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human osteosarcoma cell line. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0005.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB7567-img0003.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.")
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0003.jpg "Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).. Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-71730-img0006.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Anti-TNF-alpha was detected using a DyLight 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0002.jpg)
![ELISA: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - ELISA results of purified Goat anti-Hamster IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 ug of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG (Blue Line), and Rat IgG (Purple Line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-71730-img0004.jpg)
![Dot Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Dot Blot for Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed). Lane 1: 100 ng Hamster IgG. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: none.Secondary Antibody: Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed) at 1:1000 dilution at RT for 1 hour. Block: Fluorescent blocking buffer at RT for 30 minutes.. Using the Texas Red format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-71730-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-71730-img0008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
