Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.

Showing Publications 1 - 6 of 6.

Publications using NBP1-72739	Applications	Species
Zhang F, Zang TM, Stevenson EM et al. Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins Proceedings of the National Academy of Sciences 2022-10-11 [PMID: 36136978]
Verma SK, Yaghoobi H, Slaine P Et al. Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes Colloids Surf B Biointerfaces 2022-05-02 [PMID: 35500531] Details: Citation using the Janelia Fluor 549 version of this antibody.
Beckedorff F, Blumenthal E, daSilva LF, et al. The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts Cell Rep 2020-07-23 [PMID: 32697989] (ICC/IF)	ICC/IF
Zhang J, Sun H, Salvi R et al. Paraquat initially damages cochlear support cells leading to anoikis-like hair cell death Hear Res 2018-03-23 [PMID: 29563067] (IF/IHC)	IF/IHC
Kant V, Gopal A, Kumar D et al. Curcurmin-induced angiogenesis hastens wound healing in diabetic rats. J Surg Res. 2014-10-17 [PMID: 25454972] (IHC-P) Details: This citation used the Biotin form of this antibody.	IHC-P
Huebner KD, Shrive NG, Frank CB. Dexamethasone inhibits inflammation and cartilage damage in a new model of post-traumatic osteoarthritis. J Orthop Res. 2014-04-01 [PMID: 24375646] (IHC-Fr) Details: This citation used the HRP form of this antibody.	IHC-Fr

Array NBP1-72739

• IgG (H+L) Antibodies