Hu,
Mu,
Rt,
Pm,
RbSpecies Glossary
Reactivity: Hu, Mu, Rt, Pm, Rb
WB, Simple Western, Flow, ICC/IF, IHC, IP, B/N, ChIP, IHC
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, B/N, ChIP, IHC
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 (NB100-56708) in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/fullsize/Apoptosis-Detection-Antibody-Pack-Western-Blot-NBP2-25080-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 (NB100-56708) in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.")
![<b>Biological Strategies Validation. </b>Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of Caspase-8 in Jurkat cells using at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blot by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. this antibody was used in this test.](http://images.novusbio.com/fullsize/Apoptosis-Detection-Antibody-Pack-Western-Blot-NBP2-25080-img0006.jpg)
![Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of cleaved PARP in staurosporin-treated Jurkat cells at various time points, using at 2 ug/ml. The band corresponding to cleaved PARP is only seen in the treated samples. anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/Apoptosis-Detection-Antibody-Pack-Western-Blot-NBP2-25080-img0007.jpg)
![Simple Western: APE Antibody [NB100-101] - Lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/APE-Antibody-Simple-Western-NB100-101-img0011.jpg "Simple Western: APE Antibody [NB100-101] - Lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: APE Antibody [NB100-101] - Analysis of APE1 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-101-img0002.jpg)
![Immunohistochemistry: APE Antibody [NB100-101] - Immunohistochemical staining of APE-ref-1 in prostate cancer.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-NB100-101-img0006.jpg)
![Western Blot: APE Antibody [NB100-101] - RKO cells were subjected to scrambled siRNA (Mock) or siRNA against TDP1 (TDP1KD) and cell lysates analyzed by immunoblotting (left). Control RKO (RKOSc siRNA) and RKO cells in which TDP1 levels were depleted (RKOTDP1 siRNA) were examined for their survival following exposure to the indicated doses of MMS, as described earlier in the text (right). Increasing the load of unrepaired methylated purines by exploiting the limited availability of TDP1 alone or in combination with canonical BER factors such as APE1 provides a new synthetic lethal setting to improve the clinical outcome of temozolomide-based cancer therapy. Image collected and cropped by CiteAb from the following publication (academic.oup.com/nar/article/42/5/3089/1051107), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-101-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - IF on HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-101-img0009.jpg)
![Western Blot: APE Antibody [NB100-101] - SH-SY5Y (nuclear extract) tested at 1:1000 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-101-img0008.jpg)
![Flow Cytometry: APE Antibody [NB100-101] - Spectral properties of Alexa Fluor(R) 488: an excitation maximum of 490 nm and an emission maximum of 525 nm. Use the Novus Spectra Viewer to identify the optimal laser(s) and filters for Alexa Fluor(R) 488 and to determine its compatibility with other fluorophores when designing a multicolor experiment. //www.novusbio.com/spectraviewer](http://images.novusbio.com/fullsize/APE-Antibody-Flow-Cytometry-NB100-101-img0013.jpg)
![Western Blot: APE Antibody [NB100-101] - Analysis of APE in human melanoma cell lysate using anti-APE antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-101-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - Detection of APE1 (Green) in HepG2 cells using NB100-101. Nuclei (Blue) are counterstained with Hoechst 33258.](http://images.novusbio.com/fullsize/APE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-101-img0007.jpg)
![Western Blot: APE Antibody - BSA Free [NB100-101] - Defective p53 activity leads to failure of the BER coordination system. (A) High-throughput microscopy analysis of TIG-1 cells transfected with plasmids expressing wild-type or mutant p53. Boxplots showing the distribution of APE1 staining intensity (in arbitrary units) in p53 low versus p53 overexpressing cells. Each p53 mutant is reported on top of the relevant plot. The dashed line highlights the median APE1 intensity in p53 low cells (N > 5000). NS: not statistically significant at P 8000). (D) Representative high-throughput immuno-fluorescence pictures showing TIG-1 cells stained for APE1 (right panel) & p53 (middle panel) after transfection with a p53 expressing plasmid. Cells that downregulating APE1 in response to p53 overexpression are marked by a contour line. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-101_rabbit-polyclonal-ape-antibody-310202415483627.jpg)
![Western Blot: APE Antibody - BSA Free [NB100-101] - Excessive AP-endonuclease activity in cells with p53 impairment leads to accumulation of genomic instability. (A) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gamma H2AX staining upon co-depletion of XRCC1 & APE1. (B) Neutral Comet assay on WI38 (SV40) fibroblasts shows accumulation of DSBs upon XRCC1-depletion & rescue after co-depletion of XRCC1 & APE1. Results are expressed as mean ± SD of three independent experiments. (C) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gamma H2AX staining after depletion of XRCC1 in presence of APE1 inhibitor III (APE1i III, 5 μM for 24 h), or AR03 (2.5 μM for 24 h). Actin was used as loading control in all western blotting experiments. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-101_rabbit-polyclonal-ape-antibody-31020241548519.jpg)
![Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.](http://images.novusbio.com/fullsize/N-OLLAS-Epitope-Tag-Kit-Western-Blot-NBK1-18296-img0003.jpg "Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.")
![Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Epitope-Tag-Kit-Western-Blot-NBK1-19033-img0003.jpg "Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Epitope-Tag-Kit-NBK1-19033-img0002.jpg)
![Western Blot: SD OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19035] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Epitope-Tag-Kit-Western-Blot-NBK1-19035-img0001.jpg "Western Blot: SD OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19035] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![SD OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19035] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Epitope-Tag-Kit-NBK1-19035-img0002.jpg)
![Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB] - VECTASTAIN(R) ABC-AP Staining Kit (Alkaline Phosphatase, Standard)](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-Immunohistochemistry-AK-5000-NB-img0010.jpg "Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB] - VECTASTAIN(R) ABC-AP Staining Kit (Alkaline Phosphatase, Standard)")
![VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-AK-5000-NB-img0011.jpg)
![VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-AK-5000-NB-img0012.jpg)
![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0003.jpg "Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).")
![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0001.jpg)
![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0002.jpg)
![Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBL1-11952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.](http://images.novusbio.com/fullsize/IL32-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11952-img0002.jpg "Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBL1-11952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.")