N OLLAS Epitope Tag Expression and Detection Kit

The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC-Fr, IP, WB.

Showing Publications 1 - 2 of 2.

Publications using NBK1-18296	Applications	Species
Idoyaga, J et al. Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. J Immunol;180(6):3647-50. 2008 Mar 15. [PMID: 18322168]
Park, SH et al. Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. Epub 2007 Nov 26. 2008 Feb 29. [PMID: 18054954] (IHC-Fr, ICC/IF, FLOW, WB, IP)	IHC-Fr, ICC/IF, FLOW, WB, IP

Array NBK1-18296

• N OLLAS Epitope Tag Kit