Apoptosis Detection Antibody Pack

Apoptosis, a form of programmed cell death, is among the most highly studied and analyzed processes in cell biology. The components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery and have central roles in the initiation and execution of apoptosis. Caspases are synthesized as inactive pro enzyme precursors (zymogens) of ~30-55 kDa with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspase activation is a hallmark of apoptotic signaling pathways. Caspases are activated early during apoptosis through proteolytic cleavage at specific asparagine residues by self-proteolysis and/or cleavage by other caspases. The asparagine resides are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases consisting of the heterotetramer p202-p102. The cleavage forms present are markers of caspase-dependent apoptosis. The reduction or loss of caspase proforms can also be a marker of caspase-dependent apoptosis. Caspase activation is followed by cleavage of a myriad of caspase substrates which contributes to the demise and eventual death of the cell. The most highly characterized caspase substrate is PARP. PARP [(ADP-ribose) polymerase] is a 113 kDa nuclear chromatin-associated enzyme that is involved in DNA repair and other cellular events. The cleavage of PARP into an 89 and 28 kDa fragment is considered indicative of functional caspase activation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.

Showing Publications 1 - 2 of 2.

Publications using NBP2-25080	Applications	Species
Jarskog LF, Gilmore JH, Glantz LA et al. Caspase-3 activation in rat frontal cortex following treatment with typical and atypical antipsychotics. Neuropsychopharmacology. 2007-01-01 [PMID: 16641945] (WB, Rat) Details: IMG-144A product citation: Rat frontal cortex tissue: WB (Figs 1a and b, Table 1), IHC (frozen), Fig 3a and b.	WB	Rat
Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004-04-01 [PMID: 15019974]

Array NBP2-25080

• Apoptosis Detection Antibody Pack