VECTASTAIN® ABC HRP Detection Kit Summary
| Description |
Features:
Orignial avidin/biotin ABC complex formation
Good sensitivity and low background
Available without ("Standard") or with biotinylated species-specific secondary antibody |
| Kit Type |
HRP Detection Kit |
Applications/Dilutions
| Dilutions |
- ELISA
- Enzyme-linked Immunosorbent Spot
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
|
| Application Notes |
Reagents A and B form the ABC-enzyme complex.
Kit contains sufficient reagents to stain approximately 1000-2000 sections.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Reagent A (2 mL)
- Reagent B (2 mL)
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species.ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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