VECTASTAIN® ELITE® ABC HRP Detection Kit Summary
| Description |
Features:
Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents. |
| Kit Type |
HRP Detection Kit |
Applications/Dilutions
| Dilutions |
- ELISA
- Enzyme-linked Immunosorbent Spot
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- In-situ Hybridization
- Western Blot
|
| Application Notes |
Kit contains sufficeint reagents to stain approximately 500-1000 tissue sections.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Reagent A (Avidin DH) - 2 ml
- Reagent B (Biotinylated Horseradish Peroxidase H) - 2 ml
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0003.jpg "Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).")
![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0001.jpg "Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.")
![Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).](http://images.novusbio.com/fullsize/VECTASTAIN-R-ELITE-R-ABC-HRP-Detection-Kit-Immunohistochemistry-Paraffin-PK-6100-NB-img0002.jpg "Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).")
