VECTASTAIN® ABC AP Detection Kit Summary
| Description |
Features:
Alkaline phosphatase enzyme detection system
Avidin/biotin technology
High sensitivity/low background
Preferred for tissues that have high endogenous peroxidase activity
Offers additional substrate choices
Used as a second label in multiple antigen labeling applications
|
| Kit Type |
AP Detection Kit |
Applications/Dilutions
| Dilutions |
- ELISA
- Enzyme-linked Immunosorbent Spot
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry-Frozen
- In-situ Hybridization
- Western Blot
|
| Application Notes |
Reagent A and B when mixed form the ABC-AP complex.
Kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm^2 blots.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Reagent A - Avidin DH (2 mL)
- Reagent B - Biotinylated AP (2 mL)
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory. The sensitivity of the VECTASTAIN ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Additional VECTASTAIN(R) ABC Products
Blogs on VECTASTAIN(R) ABC
![Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB] - VECTASTAIN(R) ABC-AP Staining Kit (Alkaline Phosphatase, Standard)](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-Immunohistochemistry-AK-5000-NB-img0010.jpg "Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB] - VECTASTAIN(R) ABC-AP Staining Kit (Alkaline Phosphatase, Standard)")
![VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-AK-5000-NB-img0011.jpg "VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]")
![VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]](http://images.novusbio.com/fullsize/VECTASTAIN-R-ABC-AP-Detection-Kit-AK-5000-NB-img0012.jpg "VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]")
